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Literature summary extracted from

  • Rowan, A.D.; Buttle, D.J.; Barrett, A.J.
    Ananain: a novel cysteine proteinase found in pineapple stem (1988), Arch. Biochem. Biophys., 267, 262-270.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.31 chicken cystatin strong Ananas comosus
3.4.22.31 L-3-carboxy-2,3-trans-epoxypropionylleucylamido(4-guanidino)butane i.e. E-64 Ananas comosus
3.4.22.32 L-3-carboxy-2,3-trans-epoxypropionylleucylamido(4-guanidino)butane irreversible Ananas comosus
3.4.22.32 additional information scarcely inhibited by chicken cystatin (amino acid sequence and implications for weak binding of cystatin) Ananas comosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.31 0.0484
-
 benzoyl-Phe-Arg-7-amido-4-methylcoumarin
-
 Ananas comosus
3.4.22.32 0.0154
-
 benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide
-
 Ananas comosus
3.4.22.32 0.0831
-
 Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide
-
 Ananas comosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.31 25000
-
 x * 25000, Ananas comosus, SDS-PAGE Ananas comosus
3.4.22.32 26000
-
 x * 26000, Ananas comosus, SDS-PAGE Ananas comosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.31 Ananas comosus
-
 commercial preparation
-
3.4.22.32 Ananas comosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.31
-
 Ananas comosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.31 stem
-
 Ananas comosus
-
3.4.22.32 stem
-
 Ananas comosus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.31 additional information
-
-
 Ananas comosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.31 azocasein + H2O
-
 Ananas comosus fragments of azocasein
-
 ?
3.4.22.31 Azocoll + H2O
-
 Ananas comosus Hydrolyzed azocoll
-
 ?
3.4.22.31 benzoyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Ananas comosus benzoyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.31 Benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide + H2O
-
 Ananas comosus Benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.31 Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide + H2O
-
 Ananas comosus Benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.31 benzyloxycarbonyl-Phe-Val-Arg-7-amido-4-methylcoumarin + H2O
-
 Ananas comosus benzyloxycarbonyl-Phe-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.31 Fibrin + H2O
-
 Ananas comosus Hydrolyzed fibrin
-
 ?
3.4.22.31 hide powder azure + H2O
-
 Ananas comosus hydrolyzed hide powder azure
-
 ?
3.4.22.31 Phosphorylase a + H2O
-
 Ananas comosus Hydrolyzed phosphorylase a
-
 ?
3.4.22.32 Benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide + H2O
-
 Ananas comosus Benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.32 Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide + H2O
-
 Ananas comosus Benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.31 ? x * 25000, Ananas comosus, SDS-PAGE Ananas comosus
3.4.22.32 ? x * 26000, Ananas comosus, SDS-PAGE Ananas comosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.31 0.321
-
 benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide
-
 Ananas comosus
3.4.22.31 7
-
 benzyloxycarbonyl-Phe-Val-Arg-7-amido-4-methylcoumarin
-
 Ananas comosus
3.4.22.32 0.13
-
 Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide
-
 Ananas comosus
3.4.22.32 27
-
 benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide
-
 Ananas comosus