Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Baines, B.S.; Brocklehurst, K.; Carey, P.R.; Jarvis, M.; Salih, E.; Storer, A.C.
    Chymopapain A. Purification and investigation by covalent chromatography and characterization by two-protonic-state reactivity-probe kinetics, steady-state kinetics and resonance Raman spectroscopy of some dithioacyl derivatives (1986), Biochem. J., 233, 119-129.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.6 0.0075
-
 benzyloxycarbonyl-Lys phenyl ester at pH 6.0 Carica papaya
3.4.22.6 0.01
-
 benzyloxycarbonyl-Lys phenyl ester at pH 6.9 and at pH 4.9 Carica papaya
3.4.22.6 5
-
 benzoyl-Arg-4-nitroanilide pH 7.4 Carica papaya
3.4.22.6 8.6
-
 benzoyl-Arg-4-nitroanilide pH 4.0 Carica papaya
3.4.22.6 13
-
 benzoyl-Arg-4-nitroanilide pH 9.2 Carica papaya
3.4.22.30 0.028
-
 benzyloxycarbonyl-Lys nitrophenyl ester pH 5.9 Carica papaya
3.4.22.30 0.035
-
 benzyloxycarbonyl-Lys nitrophenyl ester
-
 Carica papaya
3.4.22.30 16
-
 benzoyl-DL-Arg 4-nitroanilide pH 9.2 Carica papaya
3.4.22.30 18
-
 benzoyl-DL-Arg 4-nitroanilide pH 4.0 Carica papaya

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.6 Carica papaya
-
-
-
3.4.22.30 Carica papaya
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.6 latex
-
 Carica papaya
-
3.4.22.30 latex
-
 Carica papaya
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.6 benzyloxycarbonyl-Lys phenyl ester + H2O
-
 Carica papaya benzyloxycarbonyl + phenol
-
 ?
3.4.22.6 additional information the abnormal catalytic site environment of chymopapain A may have consequences for the acylation step of the catalytic act, does not pertub the conformation of the bound acyl group at the acyl-enzyme-intermediate stage of catalysis Carica papaya ?
-
 ?
3.4.22.6 N-benzoyl-Arg-4-nitroanilide + H2O
-
 Carica papaya N-benzoyl-Arg + 4-nitroaniline
-
 ?
3.4.22.30 benzyloxycarbonyl-Lys nitrophenyl ester + H2O
-
 Carica papaya ?
-
 ?
3.4.22.30 N-Benzoyl-Arg 4-nitroanilide + H2O
-
 Carica papaya ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.6 0.003
-
 benzoyl-Arg-4-nitroanilide pH 4.0 and pH 7.4 Carica papaya
3.4.22.6 0.05
-
 benzoyl-Arg-4-nitroanilide pH 9.2 Carica papaya
3.4.22.6 14
-
 benzyloxycarbonyl-Lys phenyl ester at pH 4.9 Carica papaya
3.4.22.6 15.5
-
 benzyloxycarbonyl-Lys phenyl ester at pH 6.0 and at pH 6.9 Carica papaya
3.4.22.30 0.14
-
 benzoyl-DL-Arg 4-nitroanilide pH 4.0 Carica papaya
3.4.22.30 0.15
-
 benzoyl-DL-Arg 4-nitroanilide pH 9.2 Carica papaya
3.4.22.30 49
-
 benzyloxycarbonyl-Lys nitrophenyl ester pH 5.3 and 5.9 Carica papaya