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Literature summary extracted from

  • Yang, S.Y.; He, X.Y.; Schulz, H.
    Glutamate 139 of the large alpha-subunit is the catalytic base in the dehydration of both D- and L-3-hydroxyacyl-coenzyme A but not in the isomerization of DELTA3,DELTA2-enoyl-coenzyme A catalyzed by the multienzyme complex of fatty acid oxidation from Escherichia coli (1995), Biochemistry, 34, 6441-6447.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
5.3.3.8 E134Q catalytic properties of the five different component enzyme activities of the alpha/Arg134Gln mutant complex show no significant changes as compared with those of the wild-type complex. In the alpha/Glu139Gln mutant complex DELTA3-cis-DELTA2-trans-enoyl-CoA isomerase shows 60% decreased turnover number and a significant increase in Km for 3-cis-tetradecenoyl-CoA Escherichia coli
5.3.3.8 E139Q The replacement of Glu165 by Gln leads to a strongly reduced enzymatic activity. Tyr150 is not involved in isomerase catalysis Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.3.8 0.006
-
 3-cis-tetradecenoyl-CoA wild-type enzyme complex Escherichia coli
5.3.3.8 0.089
-
 3-cis-tetradecenoyl-CoA mutant enzyme complex alpha/Glu139Gln Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.8 Escherichia coli
-
 wild-type and mutant enzymes alpha/Glu139Gln and alphaArg134Gln with mutations in the large alpha-subunit
-
5.3.3.8 Escherichia coli
-
 multienzyme complex which exhibits activity of EC 4.2.1.17, EC 1.1.1.35, EC 2.3.1.16, EC 5.1.2.3 and EC 5.3.3.8
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.3.8 partial, multienzyme complex of fatty acid oxidation Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.3.8 3-cis-Tetradecenoyl-CoA
-
 Escherichia coli 2-trans-Tetradecenoyl-CoA
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.3.8 25
-
 3-cis-tetradecenoyl-CoA mutant enzyme complex alpha/Glu139Gln Escherichia coli
5.3.3.8 63
-
 3-cis-tetradecenoyl-CoA wild-type enzyme complex Escherichia coli