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Literature summary extracted from
- Purification and characterization of leader peptidase from Escherichia coli (1983), Methods Enzymol., 97, 40-46.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.21.89 | Stable at low ionic strength in solutions of nonionic detergents at low temperature | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.89 | Carboxyphenanthroline | - |
Escherichia coli |  |
| 3.4.21.89 | cholate | - |
Escherichia coli | |
| 3.4.21.89 | deoxycholate | - |
Escherichia coli | |
| 3.4.21.89 | dinitrophenol | - |
Escherichia coli | |
| 3.4.21.89 | Mg2+ | above 1 mM | Escherichia coli | |
| 3.4.21.89 | NEM | - |
Escherichia coli | |
| 3.4.21.89 | SDS | - |
Escherichia coli | |
| 3.4.21.89 | sodium chloride | above 160 mM | Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.89 | 37000 | - |
1 * 37000, SDS-PAGE | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.89 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.89 | - |
Escherichia coli |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 3.4.21.89 | even after boiling in SDS, by dilution in Triton X-100 | Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.89 | additional information | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.89 | M13 phage procoat protein + H2O | hydrolysis of a single-Ala-+-Ala- bond, the term-+- depicts the point of cleavage | Escherichia coli | Free signal peptide + coat protein | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.21.89 | monomer | 1 * 37000, SDS-PAGE | Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.89 | additional information | - |
pI: 6.9 | Escherichia coli |
| 3.4.21.89 | 8 | 9 | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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