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Literature summary extracted from
- Omptin: an Escherichia coli outer membrane proteinase that activates plasminogen (1994), Methods Enzymol., 244, 384-399.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.23.49 | - |
Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.23.49 | arginine | - |
Escherichia coli |  |
| 3.4.23.49 | benzamidine | - |
Escherichia coli | |
| 3.4.23.49 | Cu2+ | - |
Escherichia coli | |
| 3.4.23.49 | diisopropylfluorophosphate | - |
Escherichia coli | |
| 3.4.23.49 | Fe2+ | - |
Escherichia coli | |
| 3.4.23.49 | NaCl | optimal activity in absence of NaCl, 80% inhibition by 0.15 M NaCl | Escherichia coli | |
| 3.4.23.49 | Zn2+ | uncompetitive, pH-dependent; ZnCl2 | Escherichia coli | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.23.49 | 0.000058 | - |
plasminogen | pH 5.0 | Escherichia coli | |
| 3.4.23.49 | 0.0036 | - |
plasminogen | pH 7.4 | Escherichia coli |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.23.49 | outer membrane | - |
Escherichia coli | 19867 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.23.49 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.23.49 | - |
Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.49 | additional information | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.49 | plasminogen + H2O | cleavage at an Arg-Val bond | Escherichia coli | heavy and light chain of plasmin + ? | - |
? | |
| 3.4.23.49 | Recombinant human gamma-interferon + H2O | cleavage between basic amino acids | Escherichia coli | ? | - |
? | |
| 3.4.23.49 | T7 RNA polymerase + H2O | cleavage at Lys-Arg173, Lys-Lys180, and Arg-Lys392 | Escherichia coli | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.49 | 45 | - |
- |
Escherichia coli |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.49 | 37 | 45 | 37°C: 40% of maximal activity, 45°C: maximal activity | Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.49 | 5 | - |
plasminogen | Escherichia coli |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.49 | 4 | 6 | 4: 95% of maximal activity, 6.0: 25% of maximal activity | Escherichia coli |
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