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Literature summary extracted from

  • Pacaud, M.; Richaud, C.
    Protease II from Escherichia coli. Purification and characterization (1975), J. Biol. Chem., 250, 7771-7779.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.21.83 Ca2+ decreases stability Escherichia coli
3.4.21.83 Repeated freezing and thawing does not affect esterase activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.83 Aromatic amidines
-
 Escherichia coli
3.4.21.83 benzamidine
-
 Escherichia coli
3.4.21.83 Co2+
-
 Escherichia coli
3.4.21.83 Fe2+
-
 Escherichia coli
3.4.21.83 Hg2+
-
 Escherichia coli
3.4.21.83 L-arginine
-
 Escherichia coli
3.4.21.83 additional information 1,10-phenanthroline; EDTA; not: chelating agents; PMSF; several natural trypsin inhibitors; sulfhydryl agents Escherichia coli
3.4.21.83 p-aminobenzamidine
-
 Escherichia coli
3.4.21.83 Zn2+
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.83 0.47
-
 tosyl-Lys-methyl ester N-benzoyl-L-Arg ethyl ester Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.83 cytoplasm
-
 Escherichia coli 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.83 Ca2+ stimulates amidase and proteolytic activities, maximal activation of 25% at concentrations above 25 mM Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.83 58000
-
 E. coli, sucrose density gradient centrifugation, gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.83 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.83
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.83 additional information
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.4.21.83 -80°C, 5 weeks, without or with 25 and 50 mM CaCl2, the remaining activities are 88%, 50% and 32% respectively Escherichia coli
3.4.21.83 -80°C, maximal stability in dilute solutions at protein concentrations no less than 0.3 mg/ml, pH 7.8 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.83 azocasein + H2O
-
 Escherichia coli ?
-
 ?
3.4.21.83 Denatured casein + H2O weak Escherichia coli ?
-
 ?
3.4.21.83 L-Phe-L-Arg 2-naphthylamide + H2O
-
 Escherichia coli ?
-
 ?
3.4.21.83 N-Benzoyl-DL-Arg 4-nitroanilide + H2O
-
 Escherichia coli N-Benzoyl-DL-Arg + 4-nitroaniline
-
 ?
3.4.21.83 N-Benzoyl-L-Arg ethyl ester + H2O
-
 Escherichia coli N-Benzoyl-L-Arg + ethanol
-
 ?
3.4.21.83 N-carbobenzoxy-L-Lys 4-nitrophenyl ester + H2O
-
 Escherichia coli N-carbobenzoxy-L-Lys + 4-nitrophenol
-
 ?
3.4.21.83 Tosyl-Lys methyl ester + H2O
-
 Escherichia coli Tosyl-Lys + benzyl alcohol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.83 monomer 1 * 58000, SDS-PAGE Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.83 40
-
 3 h, stable Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.83 7.5 9 N-benzoyl-DL-arginine 4-nitroanilide Escherichia coli
3.4.21.83 7.6 8.9 azocasein Escherichia coli