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Literature summary extracted from
- The coagulant enzyme from Bothrops atrox venom (batroxobin) (1976), Methods Enzymol., 45, 214-223.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.74 | imidazole | activation | Bothrops atrox |  |
| 3.4.21.74 | imidazole | activation | Bothrops asper | |
| 3.4.21.74 | imidazole | activation | Bothrops moojeni | |
| 3.4.21.74 | imidazole | activation | Bothrops marajoensis | |
| 3.4.21.74 | additional information | no activation by phenol | Bothrops atrox | |
| 3.4.21.74 | additional information | no activation by phenol | Bothrops asper | |
| 3.4.21.74 | additional information | no activation by phenol | Bothrops moojeni | |
| 3.4.21.74 | additional information | no activation by phenol | Bothrops marajoensis |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.21.74 | Stable to repeated freeze-thawing | Bothrops moojeni |
| 3.4.21.74 | Stable to repeated freeze-thawing | Bothrops marajoensis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.74 | diisopropyl fluorophosphate | 2.5 mM, pH 8 | Bothrops asper | |
| 3.4.21.74 | diisopropyl fluorophosphate | 2.5 mM, pH 8 | Bothrops atrox | |
| 3.4.21.74 | diisopropyl fluorophosphate | 2.5 mM, pH 8 | Bothrops marajoensis | |
| 3.4.21.74 | diisopropyl fluorophosphate | 2.5 mM, pH 8 | Bothrops moojeni | |
| 3.4.21.74 | additional information | no inhibition by SDS, iodoacetamide, heparin, heparinoids, hirudin, aprotinin, epsilon-aminocaproic acid, tranexamic acid | Bothrops asper | |
| 3.4.21.74 | additional information | no inhibition by SDS, iodoacetamide, heparin, heparinoids, hirudin, aprotinin, epsilon-aminocaproic acid, tranexamic acid | Bothrops atrox | |
| 3.4.21.74 | additional information | no inhibition by SDS, iodoacetamide, heparin, heparinoids, hirudin, aprotinin, epsilon-aminocaproic acid, tranexamic acid | Bothrops marajoensis | |
| 3.4.21.74 | additional information | no inhibition by SDS, iodoacetamide, heparin, heparinoids, hirudin, aprotinin, epsilon-aminocaproic acid, tranexamic acid | Bothrops moojeni | |
| 3.4.21.74 | Soybean trypsin inhibitor | not | Bothrops asper | |
| 3.4.21.74 | Soybean trypsin inhibitor | not | Bothrops atrox | |
| 3.4.21.74 | Soybean trypsin inhibitor | not | Bothrops marajoensis | |
| 3.4.21.74 | Soybean trypsin inhibitor | not | Bothrops moojeni |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.21.74 | soluble | - |
Bothrops atrox | - |
- |
| 3.4.21.74 | soluble | - |
Bothrops asper | - |
- |
| 3.4.21.74 | soluble | - |
Bothrops moojeni | - |
- |
| 3.4.21.74 | soluble | - |
Bothrops marajoensis | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.74 | 32000 | - |
1 * 32000, Bothrops asper, SDS-PAGE | Bothrops asper |
| 3.4.21.74 | 32000 | - |
1 * 32000, Bothrops asper, SDS-PAGE | Bothrops moojeni |
| 3.4.21.74 | 32000 | - |
1 * 32000, Bothrops asper, SDS-PAGE | Bothrops marajoensis |
| 3.4.21.74 | 36000 | - |
1 * 36000, Bothrops moojeni, SDS-PAGE | Bothrops asper |
| 3.4.21.74 | 36000 | - |
1 * 36000, Bothrops moojeni, SDS-PAGE | Bothrops moojeni |
| 3.4.21.74 | 36000 | - |
1 * 36000, Bothrops moojeni, SDS-PAGE | Bothrops marajoensis |
| 3.4.21.74 | 37000 | - |
Bothrops moojeni, ultracentrifugation | Bothrops moojeni |
| 3.4.21.74 | 41500 | - |
1 * 41500, Bothrops marajoensis, SDS-PAGE | Bothrops asper |
| 3.4.21.74 | 41500 | - |
1 * 41500, Bothrops marajoensis, SDS-PAGE | Bothrops moojeni |
| 3.4.21.74 | 41500 | - |
1 * 41500, Bothrops marajoensis, SDS-PAGE | Bothrops marajoensis |
| 3.4.21.74 | 43000 | - |
Bothrops marajoensis, ultracentrifugation | Bothrops marajoensis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.74 | Bothrops asper | - |
Bothrops atrox variety | - |
| 3.4.21.74 | Bothrops atrox | - |
South American pit viper, formerly EC 3.4.21.29, different varieties | - |
| 3.4.21.74 | Bothrops marajoensis | - |
Bothrops atrox variety | - |
| 3.4.21.74 | Bothrops moojeni | - |
Bothrops atrox variety | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.21.74 | glycoprotein | single-chain glycopeptide | Bothrops asper |
| 3.4.21.74 | glycoprotein | single-chain glycopeptide | Bothrops moojeni |
| 3.4.21.74 | glycoprotein | single-chain glycopeptide | Bothrops marajoensis |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.74 | Bothrops atrox variety | Bothrops asper |
| 3.4.21.74 | Bothrops atrox variety | Bothrops moojeni |
| 3.4.21.74 | Bothrops atrox variety | Bothrops marajoensis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.21.74 | venom | - |
Bothrops atrox | - |
| 3.4.21.74 | venom | - |
Bothrops asper | - |
| 3.4.21.74 | venom | - |
Bothrops moojeni | - |
| 3.4.21.74 | venom | - |
Bothrops marajoensis | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.74 | additional information | - |
- |
Bothrops atrox |
| 3.4.21.74 | additional information | - |
- |
Bothrops asper |
| 3.4.21.74 | additional information | - |
- |
Bothrops moojeni |
| 3.4.21.74 | additional information | - |
- |
Bothrops marajoensis |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.21.74 | 4°C, in dilute solution in physiological saline, pH 6, containing 0.02% gelatin and 0.3% of chlorobutol, enzyme isolated from Bothrops moojeni and Bothrops marajoensis, more than a year | Bothrops moojeni |
| 3.4.21.74 | 4°C, in dilute solution in physiological saline, pH 6, containing 0.02% gelatin and 0.3% of chlorobutol, enzyme isolated from Bothrops moojeni and Bothrops marajoensis, more than a year | Bothrops marajoensis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.74 | Benzoyl-Phe-Val-Arg 4-nitroanilide + H2O | - |
Bothrops atrox | ? | - |
? | |
| 3.4.21.74 | Benzoyl-Phe-Val-Arg 4-nitroanilide + H2O | - |
Bothrops asper | ? | - |
? | |
| 3.4.21.74 | Benzoyl-Phe-Val-Arg 4-nitroanilide + H2O | - |
Bothrops moojeni | ? | - |
? | |
| 3.4.21.74 | Benzoyl-Phe-Val-Arg 4-nitroanilide + H2O | - |
Bothrops marajoensis | ? | - |
? | |
| 3.4.21.74 | Dansyl-Gly-Gly-Val-Arg-Gly methyl ester + H2O | - |
Bothrops atrox | ? | - |
? | |
| 3.4.21.74 | Dansyl-Gly-Gly-Val-Arg-Gly methyl ester + H2O | - |
Bothrops asper | ? | - |
? | |
| 3.4.21.74 | Dansyl-Gly-Gly-Val-Arg-Gly methyl ester + H2O | - |
Bothrops moojeni | ? | - |
? | |
| 3.4.21.74 | Dansyl-Gly-Gly-Val-Arg-Gly methyl ester + H2O | - |
Bothrops marajoensis | ? | - |
? | |
| 3.4.21.74 | Fibrinogen + H2O | splits fibrinopeptide A from Aalpha-chains by hydrolysis of Arg-Gly bond, but does not split this bond in Bbeta-chain | Bothrops atrox | ? | - |
? | |
| 3.4.21.74 | Fibrinogen + H2O | splits fibrinopeptide A from Aalpha-chains by hydrolysis of Arg-Gly bond, but does not split this bond in Bbeta-chain | Bothrops asper | ? | - |
? | |
| 3.4.21.74 | Fibrinogen + H2O | splits fibrinopeptide A from Aalpha-chains by hydrolysis of Arg-Gly bond, but does not split this bond in Bbeta-chain | Bothrops moojeni | ? | - |
? | |
| 3.4.21.74 | Fibrinogen + H2O | splits fibrinopeptide A from Aalpha-chains by hydrolysis of Arg-Gly bond, but does not split this bond in Bbeta-chain | Bothrops marajoensis | ? | - |
? | |
| 3.4.21.74 | Fibrinogen + H2O | human fibrinogen is a better substrate than rabbit fibrinogen | Bothrops atrox | ? | - |
? | |
| 3.4.21.74 | Fibrinogen + H2O | human fibrinogen is a better substrate than rabbit fibrinogen | Bothrops asper | ? | - |
? | |
| 3.4.21.74 | Fibrinogen + H2O | human fibrinogen is a better substrate than rabbit fibrinogen | Bothrops moojeni | ? | - |
? | |
| 3.4.21.74 | Fibrinogen + H2O | human fibrinogen is a better substrate than rabbit fibrinogen | Bothrops marajoensis | ? | - |
? | |
| 3.4.21.74 | Human blood coagulation factor XIII + H2O | activation, Bothrops asper and Bothrops moojeni, but not Bothrops majaroensis enzyme | Bothrops asper | Activated human blood coagulation factor XIII | - |
? | |
| 3.4.21.74 | Human blood coagulation factor XIII + H2O | activation, Bothrops asper and Bothrops moojeni, but not Bothrops majaroensis enzyme | Bothrops moojeni | Activated human blood coagulation factor XIII | - |
? | |
| 3.4.21.74 | additional information | no activation of human factor VIII | Bothrops marajoensis | ? | - |
? | |
| 3.4.21.74 | additional information | no activation of plasminogen | Bothrops atrox | ? | - |
? | |
| 3.4.21.74 | additional information | no activation of plasminogen | Bothrops asper | ? | - |
? | |
| 3.4.21.74 | additional information | no activation of plasminogen | Bothrops moojeni | ? | - |
? | |
| 3.4.21.74 | additional information | no activation of plasminogen | Bothrops marajoensis | ? | - |
? | |
| 3.4.21.74 | additional information | clot formation with human plasma or bovine fibrinogen as substrate | Bothrops atrox | ? | - |
? | |
| 3.4.21.74 | additional information | clot formation with human plasma or bovine fibrinogen as substrate | Bothrops asper | ? | - |
? | |
| 3.4.21.74 | additional information | clot formation with human plasma or bovine fibrinogen as substrate | Bothrops moojeni | ? | - |
? | |
| 3.4.21.74 | additional information | clot formation with human plasma or bovine fibrinogen as substrate | Bothrops marajoensis | ? | - |
? | |
| 3.4.21.74 | additional information | fibrinogen beta-chain | Bothrops atrox | ? | - |
? | |
| 3.4.21.74 | additional information | fibrinogen beta-chain | Bothrops asper | ? | - |
? | |
| 3.4.21.74 | additional information | fibrinogen beta-chain | Bothrops moojeni | ? | - |
? | |
| 3.4.21.74 | additional information | fibrinogen beta-chain | Bothrops marajoensis | ? | - |
? | |
| 3.4.21.74 | Nalpha-Benzoyl-L-Arg ethyl ester + H2O | - |
Bothrops atrox | Nalpha-Benzoyl-L-Arg + ethanol | - |
? | |
| 3.4.21.74 | Nalpha-Benzoyl-L-Arg ethyl ester + H2O | - |
Bothrops asper | Nalpha-Benzoyl-L-Arg + ethanol | - |
? | |
| 3.4.21.74 | Nalpha-Benzoyl-L-Arg ethyl ester + H2O | - |
Bothrops moojeni | Nalpha-Benzoyl-L-Arg + ethanol | - |
? | |
| 3.4.21.74 | Nalpha-Benzoyl-L-Arg ethyl ester + H2O | - |
Bothrops marajoensis | Nalpha-Benzoyl-L-Arg + ethanol | - |
? | |
| 3.4.21.74 | Tosyl-L-arginine methyl ester + H2O | - |
Bothrops atrox | Tosyl-L-arginine + methanol | - |
? | |
| 3.4.21.74 | Tosyl-L-arginine methyl ester + H2O | - |
Bothrops asper | Tosyl-L-arginine + methanol | - |
? | |
| 3.4.21.74 | Tosyl-L-arginine methyl ester + H2O | - |
Bothrops moojeni | Tosyl-L-arginine + methanol | - |
? | |
| 3.4.21.74 | Tosyl-L-arginine methyl ester + H2O | - |
Bothrops marajoensis | Tosyl-L-arginine + methanol | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.21.74 | monomer | 1 * 32000, Bothrops asper, SDS-PAGE | Bothrops asper |
| 3.4.21.74 | monomer | 1 * 32000, Bothrops asper, SDS-PAGE | Bothrops moojeni |
| 3.4.21.74 | monomer | 1 * 32000, Bothrops asper, SDS-PAGE | Bothrops marajoensis |
| 3.4.21.74 | monomer | 1 * 36000, Bothrops moojeni, SDS-PAGE | Bothrops asper |
| 3.4.21.74 | monomer | 1 * 36000, Bothrops moojeni, SDS-PAGE | Bothrops moojeni |
| 3.4.21.74 | monomer | 1 * 36000, Bothrops moojeni, SDS-PAGE | Bothrops marajoensis |
| 3.4.21.74 | monomer | 1 * 41500, Bothrops marajoensis, SDS-PAGE | Bothrops asper |
| 3.4.21.74 | monomer | 1 * 41500, Bothrops marajoensis, SDS-PAGE | Bothrops moojeni |
| 3.4.21.74 | monomer | 1 * 41500, Bothrops marajoensis, SDS-PAGE | Bothrops marajoensis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.74 | Batroxobin | - |
Bothrops atrox |
| 3.4.21.74 | Batroxobin | - |
Bothrops asper |
| 3.4.21.74 | Batroxobin | - |
Bothrops moojeni |
| 3.4.21.74 | Batroxobin | - |
Bothrops marajoensis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.74 | 37 | - |
assay at | Bothrops atrox |
| 3.4.21.74 | 37 | - |
assay at | Bothrops asper |
| 3.4.21.74 | 37 | - |
assay at | Bothrops moojeni |
| 3.4.21.74 | 37 | - |
assay at | Bothrops marajoensis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.74 | 100 | - |
1 h, in glycerol, stable | Bothrops moojeni |
| 3.4.21.74 | 100 | - |
1 h, in glycerol, stable | Bothrops marajoensis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.74 | additional information | - |
pI: 6.6 (Bothrops moojeni) | Bothrops atrox |
| 3.4.21.74 | additional information | - |
pI: 6.6 (Bothrops moojeni) | Bothrops asper |
| 3.4.21.74 | additional information | - |
pI: 6.6 (Bothrops moojeni) | Bothrops moojeni |
| 3.4.21.74 | additional information | - |
pI: 6.6 (Bothrops moojeni) | Bothrops marajoensis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.74 | 2.5 | 9 | several h stable at 20°C | Bothrops moojeni |
| 3.4.21.74 | 2.5 | 9 | several h stable at 20°C | Bothrops marajoensis |
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