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Literature summary extracted from

  • Hilz, H.; Wiegers, U.; Adamietz, P.
    Stimulation of proteinase K action by denaturing agents: application to the isolation of nucleic acids and the degradation of masked proteins (1975), Eur. J. Biochem., 56, 103-108.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.64 SDS stimulates hydrolysis of serum albumin in a dose-dependent manner, caused primarily by denaturation of the protein substrate, inactivates with an oligopeptide as substrate Parengyodontium album

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.64 SDS stimulates hydrolysis of serum albumin in a dose-dependent manner, caused primarily by denaturation of the protein substrate, inactivates with an oligopeptide as substrate Parengyodontium album
3.4.21.64 Urea stimulates hydrolysis of serum albumin in a dose-dependent manner, caused primarily by denaturation of the protein substrate Parengyodontium album

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.64 Parengyodontium album
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-
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.64 commercial preparation from Merck Parengyodontium album
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.64 additional information the combined action of detergent and proteinase K is effective in degrading `masked' proteins in a poly(adenosine diphosphoribose) preparation which cannot be attacked by the proteinase alone Parengyodontium album ?
-
 ?
3.4.21.64 Serum albumin + H2O
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 Parengyodontium album Hydrolyzed serum albumin
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 ?