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Literature summary extracted from

  • Morihara, K.; Tsuzuki, H.
    Specificity of proteinase K from Tritirachium album Limber for synthetic peptides (1975), Agric. Biol. Chem., 39, 1489-1492.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.64 Chloromethyl ketone derivatives e.g. carboxybenzoyl-Ala-Gly-PheCH2Cl, carboxybenzoyl-Ala-PheCH2Cl Parengyodontium album

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.64 additional information
-
additional information
-
Parengyodontium album
3.4.21.64 0.8
-
acetyl-(Ala)2-Phe methyl ester
-
Parengyodontium album
3.4.21.64 0.9
-
carboxybenzoyl-(Ala)2-Lys methyl ester
-
Parengyodontium album
3.4.21.64 1
-
acetyl-(Ala)2-Ala methyl ester
-
Parengyodontium album
3.4.21.64 3
-
carboxybenzoyl-L-Ala-L-Lys methyl ester carboxybenzoyl-Phe-Lys methyl ester Parengyodontium album
3.4.21.64 3.3
-
benzoyl-L-Arg ethyl ester
-
Parengyodontium album
3.4.21.64 5.6
-
acetyl-L-Trp ethyl ester
-
Parengyodontium album
3.4.21.64 8.8
-
Acetyl-Tyr ethyl ester
-
Parengyodontium album
3.4.21.64 9
-
carboxybenzoyl-Leu-Lys methyl ester
-
Parengyodontium album
3.4.21.64 12
-
acetyl-L-Phe ethyl ester
-
Parengyodontium album
3.4.21.64 19
-
carboxybenzoyl-Gly-Lys methyl ester
-
Parengyodontium album
3.4.21.64 20
-
carboxybenzoyl-D-Ala-Lys methyl ester
-
Parengyodontium album
3.4.21.64 21
-
carboxybenzoyl-L-Lys methyl ester
-
Parengyodontium album
3.4.21.64 26
-
acetyl-L-Val methyl ester
-
Parengyodontium album
3.4.21.64 30
-
acetyl-L-Leu methyl ester
-
Parengyodontium album
3.4.21.64 45
-
acetyl-L-Ala methyl ester
-
Parengyodontium album

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.64 Parengyodontium album
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.64 commercial preparation from Merck Parengyodontium album
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.64 acetyl-(Ala)2-Ala methyl ester + H2O
-
Parengyodontium album ?
-
?
3.4.21.64 acetyl-(Ala)2-Phe methyl ester + H2O
-
Parengyodontium album ?
-
?
3.4.21.64 acetyl-L-Ala methyl ester + H2O
-
Parengyodontium album acetyl-L-Ala + methanol
-
?
3.4.21.64 acetyl-L-Leu methyl ester + H2O
-
Parengyodontium album acetyl-L-Leu + methanol
-
?
3.4.21.64 acetyl-L-Phe ethyl ester + H2O
-
Parengyodontium album acetyl-L-Phe + ethanol
-
?
3.4.21.64 acetyl-L-Trp ethyl ester + H2O
-
Parengyodontium album acetyl-L-Trp + ethanol
-
?
3.4.21.64 acetyl-L-Val methyl ester + H2O
-
Parengyodontium album acetyl-L-Val + methanol
-
?
3.4.21.64 Acetyl-Tyr ethyl ester + H2O
-
Parengyodontium album ?
-
?
3.4.21.64 Benzoyl-L-Arg ethyl ester + H2O
-
Parengyodontium album Benzoyl-L-Arg + ethanol
-
?
3.4.21.64 carboxybenzoyl-(Ala)2-Lys methyl ester + H2O
-
Parengyodontium album carboxybenzoyl-(Ala)2-Lys + methanol
-
?
3.4.21.64 carboxybenzoyl-D-Ala-L-Lys methyl ester + H2O
-
Parengyodontium album carboxybenzoyl-D-Ala-L-Lys + methanol
-
?
3.4.21.64 carboxybenzoyl-Gly-Lys methyl ester + H2O
-
Parengyodontium album carboxybenzoyl-Gly-Lys + methanol
-
?
3.4.21.64 carboxybenzoyl-L-Ala-L-Lys methyl ester + H2O
-
Parengyodontium album carboxybenzoyl-L-Ala-L-Lys + methanol
-
?
3.4.21.64 carboxybenzoyl-L-Lys methyl ester + H2O
-
Parengyodontium album carboxybenzoyl-L-Lys + methanol
-
?
3.4.21.64 carboxybenzoyl-Leu-Lys methyl ester + H2O
-
Parengyodontium album carboxybenzoyl-Leu-Lys + methanol
-
?
3.4.21.64 additional information
-
Parengyodontium album ?
-
?
3.4.21.64 N-Acetylated amino acid esters + H2O
-
Parengyodontium album ?
-
?
3.4.21.64 N-Acetylated peptide esters + H2O
-
Parengyodontium album ?
-
?
3.4.21.64 Synthetic peptide substrates + H2O primarily specific against aromatic or hydrophobic amino acid residues at the carboxyl side of the splitting point, activity is markedly promoted by elongating the peptide chain to the N-terminal from the splitting point Parengyodontium album ?
-
?