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Literature summary extracted from

  • Mizuno, K.; Nakamura, T.; Oshima, T.; Tanaka, S.; Matsuo, H.
    Characterization of KEX2-encoded endopeptidase from yeast Saccharomyces cerevisiae (1989), Biochem. Biophys. Res. Commun., 159, 305-311.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.61 cloned KEX2 gene introduced into the kex2 mutant cells and the KEX2 gene product expressed in these cells Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.61 diisopropyl fluorophosphate
-
 Saccharomyces cerevisiae
3.4.21.61 HgCl2
-
 Saccharomyces cerevisiae
3.4.21.61 leupeptin
-
 Saccharomyces cerevisiae
3.4.21.61 p-chloromercuribenzoate
-
 Saccharomyces cerevisiae
3.4.21.61 pepstatin A
-
 Saccharomyces cerevisiae
3.4.21.61 phenylmethylsulfonyl fluoride
-
 Saccharomyces cerevisiae
3.4.21.61 ZnCl2
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.61 Ca2+ required Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.61 Saccharomyces cerevisiae
-
 cloned KEX2 gene introduced into the kex2 mutant cells and the KEX2 gene product expressed in these cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.61 partial Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.61 N-tert-butyloxycarbonyl-Gly-Lys-Arg 4-methylcoumarin 7-amide + H2O 59.2% of the activity with tert-butyloxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide Saccharomyces cerevisiae N-tert-butyloxycarbonyl-Gly-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.61 Protein + H2O specificity towards the carbonyl side of Lys-Arg, Arg-Arg and Pro-Arg sequences Saccharomyces cerevisiae ?
-
 ?
3.4.21.61 tert-Butyloxycarbonyl-Ala-Pro-Arg 4-methylcoumarin 7-amide + H2O 17% of the activity with tert-butyloxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide Saccharomyces cerevisiae ?
-
 ?
3.4.21.61 tert-butyloxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide + H2O
-
 Saccharomyces cerevisiae tert-butyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.61 tert-Butyloxycarbonyl-Leu-Arg-Arg 4-methylcoumarin 7-amide + H2O 116% of the activity with tert-butyloxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide Saccharomyces cerevisiae ?
-
 ?
3.4.21.61 tert-Butyloxycarbonyl-Leu-Lys-Arg 4-methylcoumarin 7-amide + H2O 92.8% of the activity with tert-butyloxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide Saccharomyces cerevisiae ?
-
 ?
3.4.21.61 tert-Butyloxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O 38% of the activity with tert-butyloxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide Saccharomyces cerevisiae ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.61 37
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.61 5.5
-
 tert-butyloxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide Saccharomyces cerevisiae