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Literature summary extracted from
- Purification, some properties and nucleotide sequence of 5-carboxymethyl-2-hydroxymuconate isomerase of Escherichia coli (1990), FEBS Lett., 266, 63-66.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.3.10 | - |
Escherichia coli |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.10 | 14000 | - |
2 * 14000, SDS-PAGE | Escherichia coli |
| 5.3.3.10 | 14044 | - |
2 * 14044, calculation from nucleotide sequence | Escherichia coli |
| 5.3.3.10 | 30000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.3.10 | 5-carboxymethyl-2-hydroxymuconate | Escherichia coli | enzyme of homoprotocatechuate catabolic pathway | ? | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.3.10 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.3.10 | - |
Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.10 | 148.3 | - |
- |
Escherichia coli |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 5.3.3.10 | 4°C, stable for at least 4 weeks | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.3.10 | 5-carboxymethyl-2-hydroxymuconate | - |
Escherichia coli | 5-carboxy-2-oxohept-3-enedioate | - |
? | |
| 5.3.3.10 | 5-carboxymethyl-2-hydroxymuconate | enzyme of homoprotocatechuate catabolic pathway | Escherichia coli | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.3.3.10 | dimer | 2 * 14044, calculation from nucleotide sequence | Escherichia coli |
| 5.3.3.10 | dimer | 2 * 14000, SDS-PAGE | Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.10 | 7.6 | - |
- |
Escherichia coli |
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Release 2023.1 (January 2023)
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