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Literature summary extracted from

  • Garnier, M.; Vacheron, M.J.; Guinand, M.; Michel, G.
    Purification and partial characterization of the extracellular gamma-D-glutamyl-(L)meso-diaminopimelate endopeptidase I, from Bacillus sphaericus NCTC 9602 (1985), Eur. J. Biochem., 148, 539-543.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.19.11 EDTA reactivation by zinc, cobalt and manganese ions Lysinibacillus sphaericus
3.4.19.11 o-phenanthroline reactivation by zinc, cobalt and manganese ions Lysinibacillus sphaericus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.19.11 0.57
-
 N-acetylmuramoyl-L-Ala-gamma-D-Glu-(L)-meso-diaminopimelic acid-(L)-D-Ala enzyme from sporulation medium Lysinibacillus sphaericus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.19.11 extracellular
-
 Lysinibacillus sphaericus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.19.11 Co2+ activates apoenzyme, maximal activation at 0.0002 mM Lysinibacillus sphaericus
3.4.19.11 Mn2+ activates apoenzyme, maximal activation at 0.0001 mM Lysinibacillus sphaericus
3.4.19.11 Zn2+ zinc enzyme Lysinibacillus sphaericus
3.4.19.11 Zn2+ activates apoenzyme Lysinibacillus sphaericus
3.4.19.11 Zn2+ zinc-binding triad His69-Glu72-His196 Lysinibacillus sphaericus
3.4.19.11 Zn2+ maximal activation at 0.0002 mM Lysinibacillus sphaericus

Organism

EC Number Organism UniProt Comment Textmining
3.4.19.11 Lysinibacillus sphaericus
-
 NCTC 9602
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.19.11
-
 Lysinibacillus sphaericus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.19.11 N-Acetylmuramoyl-L-Ala-gamma-D-Glu-(L)-meso-diaminopimelic acid-(L)-D-Ala + H2O
-
 Lysinibacillus sphaericus N-Acetylmuramoyl-L-Ala-gamma-D-Glu + meso-diaminopimelic acid-(L)-D-Ala
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.19.11 37
-
 stable for 45 days Lysinibacillus sphaericus
3.4.19.11 55
-
 10 min, 2% loss of activity Lysinibacillus sphaericus
3.4.19.11 65
-
 10 min, 3% loss of activity Lysinibacillus sphaericus
3.4.19.11 75
-
 10 min, 13% loss of activity Lysinibacillus sphaericus
3.4.19.11 80
-
-
 Lysinibacillus sphaericus
3.4.19.11 85
-
 10 min, 88% loss of activity Lysinibacillus sphaericus
3.4.19.11 95
-
 10 min, 99% loss of activity Lysinibacillus sphaericus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.19.11 additional information
-
 pI: 5.4 Lysinibacillus sphaericus