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Literature summary extracted from

  • Meinnel, T.; Mechulam, Y.; Dardel, F.; Schmitter, J.M.; Hountondji, C.; Brunie, S.; Dessen, P.; Fayat, G.; Blanquet, S.
    Methionyl-tRNA synthetase from E. coli - A review (1990), Biochimie, 72, 625-632.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.10
-
 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.10 truncated form Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.10 K335Q mutants produced by site-directed mutagenesis, Lys335-Gln substitution results in a complete loss of activity, similar loss of activity is observed when Lys335 is changed into alanine, glutamic acid, or arginine Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.10 Periodate-oxidized ATP
-
 Escherichia coli
6.1.1.10 Periodate-oxidized tRNA
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.10 additional information
-
 additional information Km-values of mutant enzymes in aminoacylation Escherichia coli
6.1.1.10 0.0009
-
 tRNAMet wild-type enzyme Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.10 Zn2+ 2 tightly bound Zn2+ per polypeptide chain, in the vicinity of the active site Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.10 76000
-
 2 * 76000 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.10 Escherichia coli
-
 wild-type enzyme and mutants produced by site-directed mutagenesis
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
6.1.1.10 facile, after unfolding with 5 M guanidine under conditions where zinc is not sequestred Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet
-
 Escherichia coli AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
6.1.1.10 additional information methionine-dependent ATP-diphosphate exchange Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.10 dimer 2 * 76000 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.10 additional information
-
 additional information turnover numbers of mutant enzymes Escherichia coli
6.1.1.10 additional information
-
 additional information in aminoacylation Escherichia coli
6.1.1.10 3.3
-
 tRNAMet wild-type enzyme Escherichia coli