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Literature summary extracted from

  • Williams, S.G.; Greenwood, J.A.; Jones, C.W.
    Molecular analysis of the lac operon encoding the binding-protein-dependent lactose transport system and beta-galactosidase in Agrobacterium radiobacter (1992), Mol. Microbiol., 6, 1755-1768.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.5.2.2 expression in Escherichia coli Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.5.2.2 ATP + H2O + lactose/out Agrobacterium tumefaciens lactose transport ADP + phosphate + lactose/in
-
 ?
7.5.2.2 ATP + H2O + lactose/out Agrobacterium tumefaciens AR50 lactose transport ADP + phosphate + lactose/in
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.5.2.2 Agrobacterium tumefaciens
-
-
-
7.5.2.2 Agrobacterium tumefaciens AR50
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
7.5.2.2 proteolytic modification LacE is synthesized as a precursor protein of 422 amino acids, the mature protein is formed by cleavage of the 28 amino acid-N-terminal region Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.5.2.2 ATP + H2O + lactose/out
-
 Agrobacterium tumefaciens ADP + phosphate + lactose/in
-
 ?
7.5.2.2 ATP + H2O + lactose/out lactose transport Agrobacterium tumefaciens ADP + phosphate + lactose/in
-
 ?
7.5.2.2 ATP + H2O + lactose/out
-
 Agrobacterium tumefaciens AR50 ADP + phosphate + lactose/in
-
 ?
7.5.2.2 ATP + H2O + lactose/out lactose transport Agrobacterium tumefaciens AR50 ADP + phosphate + lactose/in
-
 ?

Subunits

EC Number Subunits Comment Organism
7.5.2.2 More lac operon encodes a binding-protein-dependent lactose transport system and beta-galactosidase: 1. lactose binding protein, lacE, MW 42992 calculated from amino acid sequence, 2. integral membrane protein LacF, 3. integral membrane protein lacG, 4. an ATP-binding protein, lacK, MW 39299 calculated from nucleotide sequence, 5. beta galactosidase, lacZ Agrobacterium tumefaciens