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Literature summary extracted from

  • Hafer, J.; Siebers, A.; Bakker, E.P.
    The high-affinity K+-translocating ATPase complex from Bacillus acidocaldarius consists of three subunits (1989), Mol. Microbiol., 3, 487-495.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.2.2.6 bafilomycin A1
-
 Alicyclobacillus acidocaldarius
7.2.2.6 Ca2+
-
 Alicyclobacillus acidocaldarius
7.2.2.6 N,N'-dicyclohexylcarbodiimide
-
 Alicyclobacillus acidocaldarius
7.2.2.6 ortho-vanadate
-
 Alicyclobacillus acidocaldarius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.2.2.6 0.002 0.003 K+
-
 Alicyclobacillus acidocaldarius
7.2.2.6 0.08
-
 ATP
-
 Alicyclobacillus acidocaldarius

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.2.2.6 Co2+
-
 Alicyclobacillus acidocaldarius
7.2.2.6 K+
-
 Alicyclobacillus acidocaldarius
7.2.2.6 Mg2+
-
 Alicyclobacillus acidocaldarius
7.2.2.6 Mn2+
-
 Alicyclobacillus acidocaldarius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.2.2.6 22000
-
 1 * 79000 + 1 * 74000 + 1 * 22000, SDS-PAGE Alicyclobacillus acidocaldarius
7.2.2.6 74000
-
 1 * 79000 + 1 * 74000 + 1 * 22000, SDS-PAGE Alicyclobacillus acidocaldarius
7.2.2.6 79000
-
 1 * 79000 + 1 * 74000 + 1 * 22000, SDS-PAGE Alicyclobacillus acidocaldarius

Organism

EC Number Organism UniProt Comment Textmining
7.2.2.6 Alicyclobacillus acidocaldarius
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.2.2.6
-
 Alicyclobacillus acidocaldarius

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.2.2.6 0.56
-
 addition of K+ Alicyclobacillus acidocaldarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.2.6 ATP + H2O + K+/out
-
 Alicyclobacillus acidocaldarius ADP + phosphate + K+/in
-
 ?
7.2.2.6 ATP + H2O + K+[side 1] ATP in form of MgATP2- Alicyclobacillus acidocaldarius ADP + phosphate + K+[side 2]
-
 ?

Subunits

EC Number Subunits Comment Organism
7.2.2.6 trimer 1 * 79000 + 1 * 74000 + 1 * 22000, SDS-PAGE Alicyclobacillus acidocaldarius