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Literature summary extracted from

  • Wilms, B.; Wiese, A.; Syldatk, C.; Mattes, R.; Altenbuchner, J.; Pietzsch, M.
    Cloning, nucleotide sequence and expression of a new L-N-carbamoylase gene from Arthrobacter aurescens DSM 3747 in E. coli (1999), J. Biotechnol., 68, 101-113.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.87 expression in Escherichia coli Paenarthrobacter aurescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.87 44000
-
 2 * 44000, SDS-PAGE Paenarthrobacter aurescens
3.5.1.87 93000
-
 gel filtration Paenarthrobacter aurescens

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.87 Paenarthrobacter aurescens
-
 strain DSM 3747
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.87 streamline DEAE, Mono Q Paenarthrobacter aurescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.87 5
-
-
 Paenarthrobacter aurescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.87 N-carbamoyl-DL-phenylalanine + H2O enzyme seems to be selective for aromatic amino acids Paenarthrobacter aurescens L-phenylalanine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-methionine + H2O L-stereospecific enzyme Paenarthrobacter aurescens L-methionine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-thienylalanine + H2O
-
 Paenarthrobacter aurescens L-thienylalanine + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-tryptophan + H2O
-
 Paenarthrobacter aurescens L-tryptophan + CO2 + NH3
-
 ?
3.5.1.87 N-carbamoyl-L-tyrosine + H2O
-
 Paenarthrobacter aurescens L-tyrosine + CO2 + NH3
-
 ?
3.5.1.87 N-formyl-DL-tryptophan + H2O
-
 Paenarthrobacter aurescens L-tryptophan + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.87 dimer 2 * 44000, SDS-PAGE Paenarthrobacter aurescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.87 50
-
-
 Paenarthrobacter aurescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.87 25 55 no loss of activity after 6 d at 37°C, 50% loss of activity after 2 h at 50°C Paenarthrobacter aurescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.87 0.9
-
 N-carbamoyl-L-methionine
-
 Paenarthrobacter aurescens
3.5.1.87 5
-
 N-formyl-DL-tryptophan
-
 Paenarthrobacter aurescens
3.5.1.87 5.1
-
 N-carbamoyl-L-tryptophan
-
 Paenarthrobacter aurescens
3.5.1.87 6.5
-
 N-carbamoyl-L-tyrosine
-
 Paenarthrobacter aurescens
3.5.1.87 16.1
-
 N-carbamoyl-L-thienylalanine
-
 Paenarthrobacter aurescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.87 8 9
-
 Paenarthrobacter aurescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.87 7 11 enzyme activity significantly decreases below, enzyme is highly active up to pH 11.0 Paenarthrobacter aurescens