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Literature summary extracted from

  • Kooo, C.W.
    Mandelamide hydrolase from Pseudomonas putida (1997), Diss. Abstr. Int. B, 58, 1865.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.86 expression in Escherichia coli Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.86 15
-
 (R)-(-)-mandelamide
-
 Pseudomonas putida
3.5.1.86 46
-
 (R)-(-)-mandelamide
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.86 53810 53830 electrospray ionization mass spectrometry Pseudomonas putida
3.5.1.86 53850
-
 matrix-assisted laser desorption ionization Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.86 Pseudomonas putida
-
 ATCC 12633
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.86 recombinant enzyme Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.86 (R)-(-)-mandelamide + H2O
-
 Pseudomonas putida (R)-(-)mandelate + NH3
-
 ?
3.5.1.86 (S)-(+)-mandelamide + H2O
-
 Pseudomonas putida (S)-(+)-mandelate + NH3
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.86 4.4
-
 (S)-(+)-mandelamide
-
 Pseudomonas putida
3.5.1.86 10.2
-
 (R)-(-)-mandelamide
-
 Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.86 6.5 8
-
 Pseudomonas putida