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Literature summary extracted from

  • Nogawa, M.; Yatsui, K.; Tomioka, A.; Okada, H.; Morikawa, Y.
    An alpha-L-arabinofuranosidase from Trichoderma reesei containing a noncatalytic xylan-binding domain (1999), Appl. Environ. Microbiol., 65, 3964-3968.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.55 L-arabinose inducer of AF activity Trichoderma reesei
3.2.1.55 sorbose inducer of AF activity Trichoderma reesei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55
-
 Streptomyces lividans
3.2.1.55 alpha-AF cDNA cloned from Trichoderma reesei RUT C-30 chromosomal DNA using PCR technique, trying to express this gene in the yeast Schizosaccharomyces pombe, expression in Saccharomyces cerevisiae Trichoderma reesei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 0.9
-
 p-nitrophenyl-alpha-L-arabinofuranoside isoenzyme AF53 Trichoderma reesei
3.2.1.55 1.1
-
 p-nitrophenyl-alpha-L-arabinofuranoside isoenzyme AF35 Trichoderma reesei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 35000
-
 AF35, SDS-PAGE Trichoderma reesei
3.2.1.55 53000
-
 AF53, SDS-PAGE Trichoderma reesei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.55 oat spelt xylan + H2O Trichoderma reesei
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Trichoderma reesei PC-3-7
-
 L-arabinose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Streptomyces lividans
-
 66strain 1326
-
3.2.1.55 Streptomyces lividans 66 1326
-
 66strain 1326
-
3.2.1.55 Trichoderma reesei
-
 PC-3-7
-
3.2.1.55 Trichoderma reesei PC-3-7
-
 PC-3-7
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55 isoenzymes AF35 and AF53 Trichoderma reesei

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.55 conidium
-
 Trichoderma reesei
-
3.2.1.55 mycelium
-
 Trichoderma reesei
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.55 0.061
-
 isoenzyme AF35, oat spelt xylan as substrate Trichoderma reesei
3.2.1.55 2.6
-
 isoenzyme AF53, oat spelt xylan as substrate Trichoderma reesei
3.2.1.55 120
-
 isoenzyme AF53 Trichoderma reesei
3.2.1.55 196
-
 isoenzyme AF35 Trichoderma reesei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 L-arabinoxylan + H2O
-
 Trichoderma reesei L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Streptomyces lividans L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Trichoderma reesei PC-3-7 L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Streptomyces lividans 66 1326 L-arabinose + ?
-
 ir
3.2.1.55 oat spelt xylan + H2O
-
 Trichoderma reesei L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Trichoderma reesei PC-3-7 L-arabinose + ?
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Trichoderma reesei p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces lividans p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Trichoderma reesei PC-3-7 p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces lividans 66 1326 p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Trichoderma reesei L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces lividans L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Trichoderma reesei PC-3-7 L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces lividans 66 1326 L-arabinose + phenol
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 60
-
-
 Trichoderma reesei
3.2.1.55 60
-
-
 Streptomyces lividans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 40
-
 stable up to Trichoderma reesei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 4
-
-
 Trichoderma reesei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.55 4 7 isoenzyme AF35 Trichoderma reesei
3.2.1.55 4.5 7 isoenzyme AF53 Trichoderma reesei