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Literature summary extracted from

  • Prince, M.A.; Friedman, B.; Gruskin, E.A.; Schrock III, R.D.; Lloyd, R.S.
    Selective metal binding to Cys-78 within endonuclease V cause an inhibition of catalytic aactivities without altering nontarget and target DNA binding (1991), J. Biol. Chem., 266, 10686-10693.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.21.7
-
 Tequatrovirus T4

Protein Variants

EC Number Protein Variants Comment Organism
3.1.21.7 C78T 100 times more activity in the presence Hg2+ or Ag+ at concentrations they are required to inhibit the wild type enzyme Tequatrovirus T4

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.7 Ag2+ 50% inhibition at 0.01 mM Tequatrovirus T4
3.1.21.7 Cu2+ 50% inhibition at 0.025 mM Tequatrovirus T4
3.1.21.7 Hg2+ 50% inhibition at 0.0006 mM, inhibition of both DNA glycosylase and apurinic nicking activities, binding capability not reduced Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.7 double-stranded plasmid DNA + H2O Tequatrovirus T4 supercoiled pBR322-DNA nicked circular DNA + linearized DNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.7 Tequatrovirus T4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.7
-
 Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.7 double-stranded plasmid DNA + H2O supercoiled pBR322-DNA Tequatrovirus T4 nicked circular DNA + linearized DNA
-
 ?