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Literature summary extracted from

  • Olesen, C.; Thomsen, K.K.; Svendsen, I.; Brandt, A.
    The glyoxysomal 3-ketoacyl-CoA thiolase precursor from Brassica napus has enzymic activity when synthesized in Escherichia coli (1997), FEBS Lett., 412, 138-140.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.16 expression in Escherichia coli of a full-length and truncated version Brassica napus
2.3.1.155 full-length cDNA of glyoxysomal 3-ketoacyl-CoA thiolase cloned and expressed in Escherichia coli Brassica napus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.16 glyoxysome
-
 Brassica napus 9514
-
2.3.1.155 glyoxysome
-
 Brassica napus 9514
-
2.3.1.155 peroxisome
-
 Rattus norvegicus 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.155 48700
-
 calculated from amino acid sequence Brassica napus
2.3.1.155 53000
-
 SDS-PAGE Brassica napus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.16 CoA + 3-oxoacyl-CoA Brassica napus enzyme catalyses the last step in the beta-oxidation cycle acyl-CoA + acetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.16 Brassica napus
-
-
-
2.3.1.155 Brassica napus
-
-
-
2.3.1.155 Rattus norvegicus
-
-
-
2.3.1.155 Saccharomyces cerevisiae
-
 yeast
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.16 side-chain modification deletion of the first 35 N-terminal residues including the conserved cysteine from the cDNA of enzyme, both recombinant enzyme have comparable activity in Escherichia coli, the N-terminal targeting sequenze is not essential for proper folding and function of the enzyme Brassica napus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.155 recombinant protein, heterologous expressed in Escherichia coli Brassica napus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.16 CoA + 3-oxoacyl-CoA
-
 Brassica napus acyl-CoA + acetyl-CoA
-
 ?
2.3.1.16 CoA + 3-oxoacyl-CoA enzyme catalyses the last step in the beta-oxidation cycle Brassica napus acyl-CoA + acetyl-CoA
-
 ?
2.3.1.155 acetoacetyl-CoA + CoA
-
 Rattus norvegicus acetyl-CoA + acetyl-CoA
-
 ?
2.3.1.155 acetoacetyl-CoA + CoA
-
 Brassica napus acetyl-CoA + acetyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.155 dimer
-
 Rattus norvegicus
2.3.1.155 dimer
-
 Saccharomyces cerevisiae