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Literature summary extracted from
- Cholesterol biosynthesis from lanosterol: regulation and purification of rat hepatic sterol 14-reductase (1995), Biochim. Biophys. Acta, 1259, 39-48.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.1.70 | cholestyramine | 11fold induction and activation by feeding 5% cholestyramine plus 0.1% lovastatin, CL-diet. 2fold induction and activation by feeding 5% cholestyramine alone | Rattus norvegicus |  |
| 1.3.1.70 | liposome | activity of purified enzyme totally dependent on the presence of liposome and NADPH | Rattus norvegicus | |
| 1.3.1.70 | lovastatin | 11fold induction and activation by feeding 5% cholestyramine plus 0.1% lovastatin, CL-diet. 4fold induction and activation by feeding 0.1% lovastatin alone | Rattus norvegicus | |
| 1.3.1.70 | additional information | the 2.5fold increase in enzymic activity due to the diurnal rhythm is completely abolished by cycloheximide | Rattus norvegicus |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.3.1.70 | enzyme very labile | Rattus norvegicus |
| 1.3.1.70 | very labile membrane-bound enzyme, totally dependent on the presence of liposome and NADPH | Rattus norvegicus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.1.70 | 25-hydroxy-cholesterol | IC50: 0.45 mM | Rattus norvegicus | |
| 1.3.1.70 | 3beta-(2-(diethylamino)ethoxy)androst-5-en-17-one | U18666A, IC50: 0.004 mM | Rattus norvegicus | |
| 1.3.1.70 | cholesterol | enzyme suppressed by feeding 5% cholesterol, 70% decrease in enzyme activity | Rattus norvegicus | |
| 1.3.1.70 | cycloheximide | the 2.5fold increase in enzymic activity due to the diurnal rhythm is completely abolished by cycloheximide | Rattus norvegicus | |
| 1.3.1.70 | miconazole | IC50: 0.15 mM | Rattus norvegicus | |
| 1.3.1.70 | additional information | kinetic pattern of inhibition; no inhibition by lovastatin, mevalonolactone, Squalestatin 1, (E)-N-ethyl-N-(6,6-dimethyl-2-hepten-4-ynyl)-3-((3,3'-bithiophen-5-yl)methoxy)benzenemethanamine, NB-598 | Rattus norvegicus | |
| 1.3.1.70 | trans-1,4-bis(2-chlorobenzylaminomethyl)cyclohexane dihydrochloride | 0.01% AY-9944: suppression and inhibition; AY-9944, competitive inhibitor, strong inhibition, Ki: 0.00026; AY-9944, direct inhibitory effects of sterol 14-reductase; hepatocyte, IC50: 0.00025 mM, microsomes, IC50: 0.0003 mM | Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.3.1.70 | additional information | - |
additional information | kinetic pattern of inhibition | Rattus norvegicus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.3.1.70 | membrane | membrane-bound | Rattus norvegicus | 16020 | - |
| 1.3.1.70 | microsome | microsomal-bound | Rattus norvegicus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.70 | 38000 | - |
2 * 38000, two equally-sized subunits, SDS-PAGE | Rattus norvegicus |
| 1.3.1.70 | 70000 | - |
fast performance liquid chromatography | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.1.70 | additional information | Rattus norvegicus | catalyzes anaerobically NADPH-dependent reduction of 14-double bond of 8,14-diene or 7,14-diene sterols that are sterol intermediates formed during C-32 demethylation in cholesterol biosynthesis from lanosterol in mammals | ? | - |
? | |
| 1.3.1.70 | additional information | Rattus norvegicus | 14-reductase has a important regulatory role in the overall cholesterol synthetic pathway | ? | - |
? | |
| 1.3.1.70 | additional information | Rattus norvegicus | important essential enzyme of cholesterol biosynthesis from lanosterol | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.1.70 | Rattus norvegicus | - |
male sprague-dawley rats | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.3.1.70 | - |
Rattus norvegicus |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.3.1.70 | 4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP+ = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH + H+ | regulation, regulatory mechanism, enzyme induction and suppression, dietary induction | Rattus norvegicus | |
| 1.3.1.70 | 4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP+ = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH + H+ | reduction of 14-double bond of conjugated DELTA8,14-sterols and DELTA7,14-sterols | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.3.1.70 | hepatocyte | - |
Rattus norvegicus | - |
| 1.3.1.70 | liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.70 | 0.0528 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.1.70 | 4,4-dimethyl-5alpha-cholesta-7,14-dien-3beta-ol + NADPH | - |
Rattus norvegicus | 4,4-dimethyl-5alpha-cholesta-7-en-3beta-ol + NADP+ | - |
? | |
| 1.3.1.70 | additional information | enzyme acts on a range of steroids with a 14(15)-double bond | Rattus norvegicus | ? | - |
? | |
| 1.3.1.70 | additional information | catalyzes anaerobically NADPH-dependent reduction of 14-double bond of 8,14-diene or 7,14-diene sterols that are sterol intermediates formed during C-32 demethylation in cholesterol biosynthesis from lanosterol in mammals | Rattus norvegicus | ? | - |
? | |
| 1.3.1.70 | additional information | 14-reductase has a important regulatory role in the overall cholesterol synthetic pathway | Rattus norvegicus | ? | - |
? | |
| 1.3.1.70 | additional information | important essential enzyme of cholesterol biosynthesis from lanosterol | Rattus norvegicus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.3.1.70 | dimer | 2 * 38000, two equally-sized subunits, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.70 | 37 | - |
assay at, anaerobically | Rattus norvegicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.1.70 | NADPH | NADPH-dependent | Rattus norvegicus | |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 1.3.1.70 | 0.0003 | - |
hepatocyte, IC50: 0.00025 mM, microsomes, IC50: 0.0003 mM | Rattus norvegicus | trans-1,4-bis(2-chlorobenzylaminomethyl)cyclohexane dihydrochloride | |
| 1.3.1.70 | 0.004 | - |
U18666A, IC50: 0.004 mM | Rattus norvegicus | 3beta-(2-(diethylamino)ethoxy)androst-5-en-17-one | |
| 1.3.1.70 | 0.15 | - |
IC50: 0.15 mM | Rattus norvegicus | miconazole | |
| 1.3.1.70 | 0.45 | - |
IC50: 0.45 mM | Rattus norvegicus | 25-hydroxy-cholesterol | |
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