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Literature summary extracted from

  • Alonso, J.M.; Garrido-Pertierra, A.
    Kinetic properties of 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase from Escherichia coli (1986), Biochimie, 68, 731-737.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.60 ATP competitive inhibition, Ki: 0.088 mM Escherichia coli
1.2.1.60 NADH competitive inhibition, Ki: 0.025 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.60 0.009
-
 5-carboxymethyl-2-hydroxymuconate semialdehyde
-
 Escherichia coli
1.2.1.60 0.029
-
 NAD+
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.60 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ Escherichia coli involved in the meta-cleavage pathway of 4-hydroxyphenylacetate 5-carboxymethyl-2-hydroxymuconate + NADH
-
 ?
1.2.1.60 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ Escherichia coli B / ATCC 11303 involved in the meta-cleavage pathway of 4-hydroxyphenylacetate 5-carboxymethyl-2-hydroxymuconate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.60 Escherichia coli
-
-
-
1.2.1.60 Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.60 to homogeneity, chromatography techniques Escherichia coli

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.60 cell culture
-
 Escherichia coli
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.60 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ involved in the meta-cleavage pathway of 4-hydroxyphenylacetate Escherichia coli 5-carboxymethyl-2-hydroxymuconate + NADH
-
 ?
1.2.1.60 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ involved in the meta-cleavage pathway of 4-hydroxyphenylacetate Escherichia coli B / ATCC 11303 5-carboxymethyl-2-hydroxymuconate + NADH
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.60 30
-
 stable for 30 min Escherichia coli
1.2.1.60 42
-
 loss of activity after 10 min Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.60 NAD+
-
 Escherichia coli