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Literature summary extracted from

  • Fawcett, T.; Garrido-Pertierra, A.; Cooper, R.A.
    5-Carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenases of Escherichia coli C and Klebsiella pneumoniae M5a1 show very high N-terminal sequence homology (1989), FEMS Microbiol. Lett., 57, 307-312.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.60 0.005
-
 5-carboxymethyl-2-hydroxymuconate semialdehyde
-
 Klebsiella pneumoniae
1.2.1.60 0.009
-
 5-carboxymethyl-2-hydroxymuconate semialdehyde
-
 Escherichia coli
1.2.1.60 0.012
-
 NAD+
-
 Klebsiella pneumoniae
1.2.1.60 0.02
-
 NAD+
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.60 52000
-
 2 * 52000, SDS-PAGE Escherichia coli
1.2.1.60 52000
-
 2 * 52000, SDS-PAGE Klebsiella pneumoniae
1.2.1.60 102000
-
 gel filtration Klebsiella pneumoniae
1.2.1.60 125000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.60 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ Escherichia coli
-
 5-carboxymethyl-2-hydroxymuconate + NADH
-
 ?
1.2.1.60 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ Klebsiella pneumoniae
-
 5-carboxymethyl-2-hydroxymuconate + NADH
-
 ?
1.2.1.60 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ Klebsiella pneumoniae M5a1
-
 5-carboxymethyl-2-hydroxymuconate + NADH
-
 ?
1.2.1.60 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ Escherichia coli C
-
 5-carboxymethyl-2-hydroxymuconate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.60 Escherichia coli
-
-
-
1.2.1.60 Escherichia coli C
-
-
-
1.2.1.60 Klebsiella pneumoniae
-
-
-
1.2.1.60 Klebsiella pneumoniae M5a1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.60 to homogeneity, chromatography techniques Escherichia coli
1.2.1.60 to homogeneity, chromatography techniques Klebsiella pneumoniae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.60 cell culture
-
 Escherichia coli
-
1.2.1.60 cell culture
-
 Klebsiella pneumoniae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.60 35
-
-
 Escherichia coli
1.2.1.60 54
-
-
 Escherichia coli
1.2.1.60 54
-
-
 Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.60 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
-
 Escherichia coli 5-carboxymethyl-2-hydroxymuconate + NADH
-
 ?
1.2.1.60 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
-
 Klebsiella pneumoniae 5-carboxymethyl-2-hydroxymuconate + NADH
-
 ?
1.2.1.60 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
-
 Klebsiella pneumoniae M5a1 5-carboxymethyl-2-hydroxymuconate + NADH
-
 ?
1.2.1.60 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+
-
 Escherichia coli C 5-carboxymethyl-2-hydroxymuconate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.60 dimer 2 * 52000, SDS-PAGE Escherichia coli
1.2.1.60 dimer 2 * 52000, SDS-PAGE Klebsiella pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.60 7.5
-
-
 Escherichia coli
1.2.1.60 7.5
-
-
 Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.60 NAD+
-
 Escherichia coli
1.2.1.60 NAD+
-
 Klebsiella pneumoniae