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Literature summary extracted from

  • Inui, H.; Ono, K.; Miyatake, K.; Nakano, Y.; Kitaoka, S.
    Purification and characterization of pyruvate:NADP+ oxidoreductase in Euglena gracilis (1987), J. Biol. Chem., 262, 9130-9135.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.51 additional information not arsenite Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.51 0.008
-
 CoA
-
 Euglena gracilis
1.2.1.51 0.03
-
 NADP+
-
 Euglena gracilis
1.2.1.51 0.21
-
 pyruvate
-
 Euglena gracilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.51 Fe 4 mol per mol subunit Euglena gracilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.51 166000
-
 2 * 166000, SDS-PAGE Euglena gracilis
1.2.1.51 309000
-
 gel filtration Euglena gracilis
1.2.1.51 309000
-
 I. methyl viologen-pyruvate dehydrogenase fragment with iron-sulfur cluster Euglena gracilis
1.2.1.51 309000
-
 two covalently linked domains Euglena gracilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.51 pyruvate + CoA + NADP+ Euglena gracilis
-
 acetyl-CoA + CO2 + NADPH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.51 Euglena gracilis
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.2.1.51 O2 leads to rapid inactivation Euglena gracilis

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.51
-
 Euglena gracilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.51 0.93
-
 3-hydroxypyruvate Euglena gracilis
1.2.1.51 2.36
-
 2-oxobutyrate Euglena gracilis
1.2.1.51 2.6
-
 oxaloacetate Euglena gracilis
1.2.1.51 14.3
-
 pyruvate Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.51 2-oxobutyrate + CoA + NADP+
-
 Euglena gracilis propionyl-CoA + CO2 + NADPH
-
 ?
1.2.1.51 3-hydroxypyruvate + CoA + NADP+
-
 Euglena gracilis hydoxyacetyl-CoA + CO2 + NADPH
-
 ?
1.2.1.51 oxaloacetate + CoA + NADP+
-
 Euglena gracilis ? + CO2 + NADPH
-
 ?
1.2.1.51 pyruvate + CoA + NADP+
-
 Euglena gracilis acetyl-CoA + CO2 + NADPH
-
 r
1.2.1.51 pyruvate + CoA + NADP+ NADP+ can be substituted by methyl viologen Euglena gracilis acetyl-CoA + CO2 + NADPH
-
 r
1.2.1.51 pyruvate + CoA + NADP+ NADP+ can be substituted by benzyl viologen Euglena gracilis acetyl-CoA + CO2 + NADPH
-
 r
1.2.1.51 pyruvate + CoA + NADP+ reverse reaction 2% of forward reaction Euglena gracilis acetyl-CoA + CO2 + NADPH
-
 r

Subunits

EC Number Subunits Comment Organism
1.2.1.51 dimer 2 * 166000, SDS-PAGE Euglena gracilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.51 40
-
-
 Euglena gracilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.51 7.5
-
-
 Euglena gracilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.51 FAD
-
 Euglena gracilis
1.2.1.51 NADP+ not NAD+ Euglena gracilis
1.2.1.51 thiamine diphosphate
-
 Euglena gracilis