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Literature summary extracted from
- Purification and properties of NADP-dependent formate dehydrogenase from Clostridium thermoaceticum, a tungsten-selenium-iron protein (1983), J. Biol. Chem., 258, 1826-1832.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.17.1.10 | azide stabilizes during purification | Moorella thermoacetica |
| 1.17.1.10 | dithionite stabilizes during purification | Moorella thermoacetica |
| 1.17.1.10 | glycerol stabilizes during purification | Moorella thermoacetica |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.17.1.10 | 0.109 | - |
formate | - |
Moorella thermoacetica |  |
| 1.17.1.10 | 0.117 | - |
NADP+ | - |
Moorella thermoacetica | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.1.10 | Iron | 36 mol of iron per mol of enzyme | Moorella thermoacetica | |
| 1.17.1.10 | selenium | 2 mol of selenium per mol of enzyme, resides in the alpha-subunit | Moorella thermoacetica | |
| 1.17.1.10 | Tungsten | 2 mol of tungsten per mol of enzyme | Moorella thermoacetica | |
| 1.17.1.10 | Tungsten | may exist as tungsten cofactor | Moorella thermoacetica | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.10 | 76000 | - |
alpha2 beta2, 2 * 96000 + 2 * 76000 SDS-PAGE | Moorella thermoacetica |
| 1.17.1.10 | 96000 | - |
alpha2 beta2, 2 * 96000 + 2 * 76000 SDS-PAGE | Moorella thermoacetica |
| 1.17.1.10 | 340000 | - |
gel filtration | Moorella thermoacetica |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.17.1.10 | Moorella thermoacetica | - |
- |
- |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 1.17.1.10 | extremely oxygen-sensitive | Moorella thermoacetica |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.17.1.10 | - |
Moorella thermoacetica |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.10 | 1050 | - |
- |
Moorella thermoacetica |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.17.1.10 | 4°C, anaerobic conditions, glycerol, ammonium sulfate, 45 days, 35% loss of activity | Moorella thermoacetica |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.17.1.10 | CO2 + NADPH | enzyme also catalyzes: 1. reversible electron transfer between methyl viologen and NADPH, 2. reduction of FMN or FAD with NADPH (caused by a contaminating protein) | Moorella thermoacetica | formate + NADP+ | - |
? | |
| 1.17.1.10 | CO2 + NADPH | reduction of FMN or FAD with NADPH caused by a contaminating protein | Moorella thermoacetica | formate + NADP+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.17.1.10 | tetramer | alpha2 beta2, 2 * 96000 + 2 * 76000 SDS-PAGE | Moorella thermoacetica |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.1.10 | NADP+ | - |
Moorella thermoacetica | |
| 1.17.1.10 | NADPH | - |
Moorella thermoacetica | |
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Release 2023.1 (January 2023)
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