EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.17.1.10 | sulfhydryl compounds | required for activity, e.g. dithiothreitol, cysteine, glutathione, thioglycolate | Moorella thermoacetica |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.17.1.10 | 2,3-Butanedione | sodium azide and NADP+ protect | Moorella thermoacetica | |
1.17.1.10 | azide | competitive inhibitor | Moorella thermoacetica | |
1.17.1.10 | cyanide | 50% inhibition at 0.01 mM, 100% inhibition at 10 mM | Moorella thermoacetica | |
1.17.1.10 | EDTA | 50% inhibition at 25 mM, 100% inhibition at 100 mM | Moorella thermoacetica | |
1.17.1.10 | hypophosphite | competitive inhibitor | Moorella thermoacetica | |
1.17.1.10 | mercaptoethanol | - |
Moorella thermoacetica | |
1.17.1.10 | additional information | enzyme is rapidly inactivated by oxygen: 93% loss of activity within 2 min | Moorella thermoacetica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.17.1.10 | 0.02 | - |
NADPH | - |
Moorella thermoacetica | |
1.17.1.10 | 0.083 | - |
formate | cosubstrate methyl viologen | Moorella thermoacetica | |
1.17.1.10 | 0.11 | - |
NADP+ | - |
Moorella thermoacetica | |
1.17.1.10 | 0.23 | - |
formate | cosubstrate NADP+ | Moorella thermoacetica | |
1.17.1.10 | 2.35 | - |
reduced methyl viologen | - |
Moorella thermoacetica | |
1.17.1.10 | 11 | - |
CO2 | - |
Moorella thermoacetica |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.17.1.10 | Molybdenum | - |
Moorella thermoacetica | |
1.17.1.10 | selenium | - |
Moorella thermoacetica | |
1.17.1.10 | Tungsten | - |
Moorella thermoacetica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.17.1.10 | 270000 | 300000 | gel filtration | Moorella thermoacetica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.1.10 | CO2 + NADPH | Moorella thermoacetica | first enzyme in pathway of reduction of CO2 to acetate: CO2 serves as electron acceptor generated during fermentation | formate + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.17.1.10 | Moorella thermoacetica | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.17.1.10 | rapid inactivation by air, thiol-iron complexes and formate stabilize | Moorella thermoacetica |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.17.1.10 | partial | Moorella thermoacetica |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.17.1.10 | 36.6 | - |
- |
Moorella thermoacetica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.1.10 | CO2 + NADPH | enzyme also catalyzes: 3. oxidation of NADPH with O2 | Moorella thermoacetica | formate + NADP+ | - |
r | |
1.17.1.10 | CO2 + NADPH | also reacts with: methyl viologen, benzyl viologen | Moorella thermoacetica | formate + NADP+ | - |
r | |
1.17.1.10 | CO2 + NADPH | first enzyme in pathway of reduction of CO2 to acetate: CO2 serves as electron acceptor generated during fermentation | Moorella thermoacetica | formate + NADP+ | - |
? | |
1.17.1.10 | formate + reduced methyl viologen | - |
Moorella thermoacetica | CO2 + oxidized methyl viologen | - |
r |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.17.1.10 | 70 | - |
formate + NADP+ | Moorella thermoacetica |
1.17.1.10 | 85 | - |
formate + methyl viologen | Moorella thermoacetica |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.17.1.10 | 40 | - |
low activity below | Moorella thermoacetica |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.17.1.10 | 70 | - |
3 h, slight loss of activity | Moorella thermoacetica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.17.1.10 | 7 | - |
broad, CO2 reduction | Moorella thermoacetica |
1.17.1.10 | 7 | 9.5 | formate oxidation | Moorella thermoacetica |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.17.1.10 | NADP+ | - |
Moorella thermoacetica | |
1.17.1.10 | NADPH | - |
Moorella thermoacetica |