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Literature summary extracted from

  • Baldoma, L.; Aguilar, J.
    Involvement of lactaldehyde dehydrogenase in several metabolic pathways of Escherichia coli K12 (1987), J. Biol. Chem., 262, 13991-13996.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.22 Ca2+ 50% inhibition at 5 mM Escherichia coli
1.2.1.22 Cu2+ 50% inhibition at 0.25 mM Escherichia coli
1.2.1.22 glyceraldehyde substrate inhibition Escherichia coli
1.2.1.22 glycolaldehyde substrate inhibition Escherichia coli
1.2.1.22 lactaldehyde substrate inhibition Escherichia coli
1.2.1.22 methylglyoxal substrate inhibition Escherichia coli
1.2.1.22 Mn2+ 50% inhibition at 10 mM Escherichia coli
1.2.1.22 p-hydroxymercuribenzoate 50% inhibition at 1.5 mM Escherichia coli
1.2.1.22 Zn2+ 50% inhibition at 0.4 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.22 0.04
-
 L-lactaldehyde
-
 Escherichia coli
1.2.1.22 0.12
-
 NAD+ + L-lactaldehyde Escherichia coli
1.2.1.22 0.15
-
 L-glyceraldehyde
-
 Escherichia coli
1.2.1.22 0.28
-
 NAD+ cosubstrate glycolaldehyde Escherichia coli
1.2.1.22 0.38
-
 glycolaldehyde
-
 Escherichia coli
1.2.1.22 1
-
 methylglyoxal
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.22 55000
-
 4 * 55000, SDS-PAGE Escherichia coli
1.2.1.22 220000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.22 (S)-lactaldehyde + NAD+ Escherichia coli general role in aldehyde oxidation, involved in several metabolic pathways (S)-lactate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.22 Escherichia coli
-
 inducible under aerobic conditions
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.22
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.22 additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.22 (S)-lactaldehyde + NAD+ general role in aldehyde oxidation, involved in several metabolic pathways Escherichia coli (S)-lactate + NADH
-
 ?
1.2.1.22 (S)-lactaldehyde + NAD+ + H2O
-
 Escherichia coli (S)-lactate + NADH + H+
-
 ir
1.2.1.22 glycolaldehyde + NAD+ + H2O
-
 Escherichia coli glycolate + NADH
-
 ir
1.2.1.22 L-glyceraldehyde + NAD+ + H2O
-
 Escherichia coli L-glycerate + NADH
-
 ir
1.2.1.22 methylglyoxal + NAD+ + H2O
-
 Escherichia coli pyruvate + NADH
-
 ir
1.2.1.22 additional information oxidizes several aldehydes, e.g. L-glyceraldehyde, glycolaldehyde, methylglyoxal Escherichia coli ?
-
 ?
1.2.1.22 additional information not: acetaldehyde, formaldehyde, propionaldehyde, succinic semialdehyde Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.22 tetramer 4 * 55000, SDS-PAGE Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.22 NAD+ four NAD-binding sites per molecule of native enzyme Escherichia coli