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Literature summary extracted from

  • Inoue, Y.; Watanabe, K.; Shimosaka, M.; Saikusa, T.; Fukuda, Y.; Murata, K.; Kimura, A.
    Metabolism of 2-oxoaldehydes in yeasts. Purification and characterization of lactaldehyde dehydrogenase from Saccharomyces cerevisiae (1985), Eur. J. Biochem., 153, 243-247.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.22 dithiothreitol slight activation Saccharomyces cerevisiae
1.2.1.22 glutathione slight activation Saccharomyces cerevisiae

General Stability

EC Number General Stability Organism
1.2.1.22 purified enzyme is extremely unstable, reactivation with halide ions e.g. Cl-, I-, Br- Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.22 Ca2+
-
 Saccharomyces cerevisiae
1.2.1.22 Cu2+
-
 Saccharomyces cerevisiae
1.2.1.22 Hg2+
-
 Saccharomyces cerevisiae
1.2.1.22 additional information iodoacetamide, Mn2+, Mg2+, Co2+, no effect at 5 mM Saccharomyces cerevisiae
1.2.1.22 N-ethylmaleimide
-
 Saccharomyces cerevisiae
1.2.1.22 Ni2+
-
 Saccharomyces cerevisiae
1.2.1.22 p-chloromercuribenzoate
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.22 2.9
-
 NAD+
-
 Saccharomyces cerevisiae
1.2.1.22 10
-
 L-lactaldehyde
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.22 Br- activates Saccharomyces cerevisiae
1.2.1.22 Cl- activates Saccharomyces cerevisiae
1.2.1.22 I- activates Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.22 40000
-
 gel filtration, SDS-PAGE Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.22 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.22
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.22 0.392
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
1.2.1.22 -20°C, pH 7.5, purified enzyme is extremely unstable, almost completely inactivated, reactivation with halide ions Cl-, I-, Br- Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.22 (S)-lactaldehyde + NAD+ + H2O
-
 Saccharomyces cerevisiae (S)-lactate + NADH + H+
-
 ?
1.2.1.22 D-lactaldehyde + NAD+ + H2O 0.2% the activity of L-lactaldehyde reduction Saccharomyces cerevisiae D-lactate + NADH + H+
-
 ?
1.2.1.22 additional information not: acetaldehyde, DL-glyceraldehyde, propionaldehyde, phenylpyruvate Saccharomyces cerevisiae ?
-
 ?
1.2.1.22 additional information specifically oxidizes L-lactaldehyde to L-lactate in presence of NAD+ Saccharomyces cerevisiae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.22 NAD-dependent lactaldehyde dehydrogenase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.22 60
-
-
 Saccharomyces cerevisiae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.1.22 additional information
-
 rapid decline in activity above 60°C Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.22 50
-
 pH 6.5, 10 min, 50% loss of activity Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.22 6.5
-
-
 Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.22 7.5
-
 4°C, stable Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.22 additional information NADP+ can not substitute for NAD+ Saccharomyces cerevisiae
1.2.1.22 NAD+
-
 Saccharomyces cerevisiae