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Literature summary extracted from

  • Kato, N.; Kano, M.; Tani, Y.; Ogata, K.
    Purification and characterization of formate dehydrogenase in a methanol-utilizing yeast, Kloeckera sp. No. 2201 (1974), Agric. Biol. Chem., 38, 111-116.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.9 CN-
-
 Kloeckera sp.
1.17.1.9 Cu2+
-
 Kloeckera sp.
1.17.1.9 Hg2+
-
 Kloeckera sp.
1.17.1.9 N3-
-
 Kloeckera sp.
1.17.1.9 Pb2+
-
 Kloeckera sp.
1.17.1.9 PCMB
-
 Kloeckera sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.9 0.1
-
 NAD+
-
 Kloeckera sp.
1.17.1.9 22
-
 formate
-
 Kloeckera sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.9 formate + NAD+ Kloeckera sp. last step of methanol oxidation system CO2 + NADH + H+
-
 ?
1.17.1.9 formate + NAD+ Kloeckera sp. 2201 last step of methanol oxidation system CO2 + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.9 Kloeckera sp.
-
 No. 2201
-
1.17.1.9 Kloeckera sp. 2201
-
 No. 2201
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.9
-
 Kloeckera sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.9 0.143
-
-
 Kloeckera sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.9 formate + NAD+
-
 Kloeckera sp. CO2 + NADH + H+
-
 ?
1.17.1.9 formate + NAD+ last step of methanol oxidation system Kloeckera sp. CO2 + NADH + H+
-
 ?
1.17.1.9 formate + NAD+
-
 Kloeckera sp. 2201 CO2 + NADH + H+
-
 ?
1.17.1.9 formate + NAD+ last step of methanol oxidation system Kloeckera sp. 2201 CO2 + NADH + H+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.1.9 50
-
-
 Kloeckera sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.17.1.9 30 60 30°C: about 40% of maximal activity, 60°C: about 80% of maximal activity Kloeckera sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.17.1.9 50
-
 10 min, stable Kloeckera sp.
1.17.1.9 58
-
 10 min, 60% loss of activity Kloeckera sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.1.9 8
-
-
 Kloeckera sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.17.1.9 5 10 pH 5.0: about 50% of maximal activity, pH 10.0: about 65% of maximal activity Kloeckera sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.9 NAD+
-
 Kloeckera sp.