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Literature summary extracted from

  • Kearny, J.J.; Sagers, R.D.
    Formate dehydrogenase from Clostridium acidiurici (1972), J. Bacteriol., 109, 152-161.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.17.1.9 instability to freezing and thawing Gottschalkia acidurici
1.17.1.9 strong inhibition by light Gottschalkia acidurici

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.9 CN- 0.1 mM NaCN, 90% inhibition Gottschalkia acidurici
1.17.1.9 EDTA 50 mM, slight inhibition Gottschalkia acidurici

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.9 0.1
-
 benzyl viologen
-
 Gottschalkia acidurici
1.17.1.9 3.1
-
 formate
-
 Gottschalkia acidurici

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.9 Gottschalkia acidurici
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.17.1.9 strong inhibition by O2 Gottschalkia acidurici

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.9
-
 Gottschalkia acidurici

Storage Stability

EC Number Storage Stability Organism
1.17.1.9 5°C, in the dark, in 0.05 M potassium phosphate buffer, pH 7, 1 mM EDTA, 5 mM 2-mercaptoethanol, 10% ammonium sulfate, stable for at least 3 weeks Gottschalkia acidurici

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.9 formate + benzyl viologen
-
 Gottschalkia acidurici CO2 + reduced benzyl viologen
-
 ?
1.17.1.9 formate + NAD+
-
 Gottschalkia acidurici CO2 + NADH + H+
-
 ir

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.1.9 7.8 8.2
-
 Gottschalkia acidurici

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.9 NAD+
-
 Gottschalkia acidurici