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Literature summary extracted from

  • Prieto, M.I.; Martin, J.; Balana-Fouce, R.; Garrido-Pertierra, A.
    Properties of gamma-aminobutyraldehyde dehydrogenase from Escherichia coli (1987), Biochimie, 69, 1161-1168.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.19 NADH competitive inhibitor with respect to NAD+ Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.19 0.0313
-
 DELTA1-pyrroline
-
 Escherichia coli
1.2.1.19 0.0538
-
 NAD+
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.19 additional information no requirement for bivalent cations Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.19 95000
-
 2 * 95000, SDS-PAGE Escherichia coli
1.2.1.19 195000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.19 4-aminobutanal + NAD+ + H2O Escherichia coli role in putrescine degradative pathway 4-aminobutanoate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.19 Escherichia coli
-
 K-12
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.19 from putrescine-grown cells, using ammonium sulfate fractionation and column chromatography on Sephacryl S-300, DEAE-Sephacel and Blue-Sepharose CL6B Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.19 additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.19 4-aminobutanal + NAD+ + H2O
-
 Escherichia coli 4-aminobutanoate + NADH + H+
-
 ?
1.2.1.19 4-aminobutanal + NAD+ + H2O role in putrescine degradative pathway Escherichia coli 4-aminobutanoate + NADH + H+
-
 ?
1.2.1.19 DELTA1-pyrroline + NAD+ + H2O
-
 Escherichia coli 4-aminobutanoate + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.2.1.19 dimer 2 * 95000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.19 75
-
 increase in dehydrogenase capacity from 25°C to 75°C Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.19
-
 35 up to 5 min, stable Escherichia coli
1.2.1.19 35
-
 unstable above Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.19 5.4
-
 in the presence of sodium citrate buffer Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.19 4 7 at pH 4.0 and pH 7.0: about 60% of activity maximum Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.19 NAD+ absolutely dependent on Escherichia coli