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Literature summary extracted from

  • Grissom, F.E.; Kahn, J.S.
    Glyceraldehyde-3-phosphate dehydrogenases from Euglena gracilis. Purification and physical and chemical characterization (1975), Arch. Biochem. Biophys., 171, 444-458.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.13 ATP activation Euglena gracilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.12 ATP 10 mM, 50% inhibition Euglena gracilis
1.2.1.12 cAMP
-
 Euglena gracilis
1.2.1.12 iodoacetate
-
 Euglena gracilis
1.2.1.12 NEM 1 mM, complete inhibition Euglena gracilis
1.2.1.12 PCMB 1 mM, complete inhibition, partially reversed by dithiothreitol; 1 mM, inhibition is partially reversed by dithiothreitol Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.12 0.0066
-
 NAD+
-
 Euglena gracilis
1.2.1.12 0.021
-
 NADH
-
 Euglena gracilis
1.2.1.12 0.081
-
 D-glyceraldehyde 3-phosphate
-
 Euglena gracilis
1.2.1.12 0.47
-
 3-phospho-D-glyceroyl phosphate
-
 Euglena gracilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.12 37000
-
 4 * 37000, SDS-PAGE Euglena gracilis
1.2.1.12 137000
-
 gel filtration Euglena gracilis
1.2.1.12 138600
-
 sucrose density gradient centrifugation Euglena gracilis
1.2.1.12 140500
-
 equilibrium sedimentation Euglena gracilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.13 3-phospho-D-glyceroyl phosphate + NADPH Euglena gracilis probable function in CO2 fixation D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.12 Euglena gracilis
-
-
-
1.2.1.13 Euglena gracilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.12
-
 Euglena gracilis
1.2.1.13
-
 Euglena gracilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.12 additional information
-
-
 Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.12 3-phospho-D-glyceroyl phosphate + NADH
-
 Euglena gracilis D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 r
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Euglena gracilis 3-phospho-D-glyceroyl phosphate + NADH
-
 r
1.2.1.13 3-phospho-D-glyceroyl phosphate + NADPH probable function in CO2 fixation Euglena gracilis D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.12 tetramer 4 * 37000, SDS-PAGE Euglena gracilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.12 30
-
-
 Euglena gracilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.12 7.9 8.9
-
 Euglena gracilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.12 NAD+
-
 Euglena gracilis
1.2.1.12 NADH cofactor Euglena gracilis
1.2.1.13 NADP+ cofactor Euglena gracilis