Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Copeland, L.; Zammit, A.
    Kinetic properties of NAD-dependent glyceraldehyde-3-phosphate dehydrogenase from the host fraction of soybean root nodules (1994), Arch. Biochem. Biophys., 312, 107-113.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.12 2,3-diphosphoglycerate
-
 Glycine max
1.2.1.12 AMP
-
 Glycine max
1.2.1.12 NADH competitive with respect to NAD+ and phosphate Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.12 additional information
-
 additional information
-
 Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.12 Glycine max
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH + H+ bi-uni-uni-uni-ping-pong mechanism in which NAD+ and phosphate interact sequentially with the enzyme, followed in turn by the release of 3-phospho-D-glyceroyl phosphate, the addition of D-glyceraldehyde-3-phosphate, and the release of NADH. At pH 7.2, NAD+ binds to the enzyme in a rapid-equilibrium fashion, whereas at pH 8.8 there is rapid-equilibrium addition of both NAD+ and phosphate to the enzyme Glycine max
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.12 nodule
-
 Glycine max
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Glycine max 3-phospho-D-glyceroyl phosphate + NADH
-
 ?

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.12 8
-
 very labile, especially above pH 8.0 Glycine max

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.12 NAD+ at pH 8.8 there is rapid-equilibrium addition of both NAD+ and phosphate to the enzyme Glycine max