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Literature summary extracted from

  • Kocha, T.; Fukuda, T.; Isobe, T.; Okuyama, T.
    Specific purification of glyceraldehyde-3-phosphate dehydrogenase by hydrophobic chromatography on immobilized colchicine (1989), Biochim. Biophys. Acta, 991, 56-61.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.12 cytosol
-
 Bos taurus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.12 35000
-
 4 * 35000, SDS-PAGE Bos taurus
1.2.1.12 142000
-
 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.12 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.12 hydrophobic chromatography on immobilized colchicine Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.12 brain
-
 Bos taurus
-
1.2.1.12 heart
-
 Bos taurus
-
1.2.1.12 muscle
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.12 195
-
 brain Bos taurus
1.2.1.12 211
-
 muscle Bos taurus
1.2.1.12 219
-
 heart Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Bos taurus 3-phospho-D-glyceroyl phosphate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.12 tetramer 4 * 35000, SDS-PAGE Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.12 NAD+
-
 Bos taurus