BRENDA - Enzyme Database

Maize glutathione-dependent formaldehyde dehydrogenase. Protein sequence and catalytic properties

Wippermann, U.; Fliegmann, J.; Bauw, G.; Langebartels, C.; Maier, K.; Sandermann, H.Jr.; Planta 208, 12-18 (1999)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.284
pyrazole
-
Canis lupus familiaris
1.1.1.284
pyrazole
-
Zea mays
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.019
-
formaldehyde
-
Canis lupus familiaris
1.1.1.284
0.02
-
formaldehyde
-
Zea mays
1.1.1.284
0.021
-
formaldehyde
-
Zea mays
1.1.1.284
0.031
-
NAD+
-
Canis lupus familiaris
1.1.1.284
0.031
-
NAD+
-
Zea mays
1.1.1.284
0.037
-
glutathione
-
Zea mays
1.1.1.284
0.062
-
glutathione
-
Canis lupus familiaris
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.284
42000
-
2 * 42000, SDS-PAGE
Zea mays
1.1.1.284
82000
-
gel filtration
Zea mays
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.284
additional information
Zea mays
the enzyme may be involved in the detoxification of long-chain lipid peroxidation products
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.284
Canis lupus familiaris
-
-
-
1.1.1.284
Zea mays
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.284
-
Canis lupus familiaris
1.1.1.284
-
Zea mays
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.284
cell suspension culture
-
Zea mays
-
1.1.1.284
lung
-
Canis lupus familiaris
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.284
4.68
-
-
Zea mays
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
10-hydroxydodecanoic acid + glutathione + NAD+
-
287891
Zea mays
?
-
-
-
?
1.1.1.284
12-hydroxydodedecanoic acid + glutathione + NAD+
-
287891
Zea mays
12-oxododecanoic acid + ?
-
-
-
?
1.1.1.284
formaldehyde + glutathione + NAD+
the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione
287891
Zea mays
S-formylglutathione + NADH + H+
-
287891
Zea mays
?
1.1.1.284
formaldehyde + glutathione + NAD+
the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione
287891
Canis lupus familiaris
S-formylglutathione + NADH + H+
-
287891
Canis lupus familiaris
?
1.1.1.284
additional information
the enzyme may be involved in the detoxification of long-chain lipid peroxidation products
287891
Zea mays
?
-
-
-
-
1.1.1.284
octanol + glutathione + NAD+
octanol-1
287891
Zea mays
?
-
-
-
?
1.1.1.284
pentanol + glutathione + NAD+
-
287891
Zea mays
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.284
dimer
2 * 42000, SDS-PAGE
Zea mays
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.284
45
-
-
Zea mays
1.1.1.284
55
-
-
Canis lupus familiaris
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.284
additional information
-
additional information
-
Zea mays
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.284
8
9.5
-
Canis lupus familiaris
1.1.1.284
8
9.5
-
Zea mays
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Canis lupus familiaris
1.1.1.284
NAD+
-
Zea mays
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Canis lupus familiaris
1.1.1.284
NAD+
-
Zea mays
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.284
pyrazole
-
Canis lupus familiaris
1.1.1.284
pyrazole
-
Zea mays
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.019
-
formaldehyde
-
Canis lupus familiaris
1.1.1.284
0.02
-
formaldehyde
-
Zea mays
1.1.1.284
0.021
-
formaldehyde
-
Zea mays
1.1.1.284
0.031
-
NAD+
-
Canis lupus familiaris
1.1.1.284
0.031
-
NAD+
-
Zea mays
1.1.1.284
0.037
-
glutathione
-
Zea mays
1.1.1.284
0.062
-
glutathione
-
Canis lupus familiaris
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.284
42000
-
2 * 42000, SDS-PAGE
Zea mays
1.1.1.284
82000
-
gel filtration
Zea mays
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.284
additional information
Zea mays
the enzyme may be involved in the detoxification of long-chain lipid peroxidation products
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.284
-
Canis lupus familiaris
1.1.1.284
-
Zea mays
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.284
cell suspension culture
-
Zea mays
-
1.1.1.284
lung
-
Canis lupus familiaris
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.284
4.68
-
-
Zea mays
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
10-hydroxydodecanoic acid + glutathione + NAD+
-
287891
Zea mays
?
-
-
-
?
1.1.1.284
12-hydroxydodedecanoic acid + glutathione + NAD+
-
287891
Zea mays
12-oxododecanoic acid + ?
-
-
-
?
1.1.1.284
formaldehyde + glutathione + NAD+
the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione
287891
Zea mays
S-formylglutathione + NADH + H+
-
287891
Zea mays
?
1.1.1.284
formaldehyde + glutathione + NAD+
the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione
287891
Canis lupus familiaris
S-formylglutathione + NADH + H+
-
287891
Canis lupus familiaris
?
1.1.1.284
additional information
the enzyme may be involved in the detoxification of long-chain lipid peroxidation products
287891
Zea mays
?
-
-
-
-
1.1.1.284
octanol + glutathione + NAD+
octanol-1
287891
Zea mays
?
-
-
-
?
1.1.1.284
pentanol + glutathione + NAD+
-
287891
Zea mays
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.284
dimer
2 * 42000, SDS-PAGE
Zea mays
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.284
45
-
-
Zea mays
1.1.1.284
55
-
-
Canis lupus familiaris
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.284
additional information
-
additional information
-
Zea mays
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.284
8
9.5
-
Canis lupus familiaris
1.1.1.284
8
9.5
-
Zea mays