BRENDA - Enzyme Database

Formaldehyde dehydrogenase

Uotila, L.; Koivusalo, M.; Methods Enzymol. 77, 314-320 (1981)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.1.1.284
NAD+ and NADH protect from denaturation by high temperatures, acid pH and storage at 0°C
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.284
ADP-ribose
-
Homo sapiens
1.1.1.284
Alpha-NAD+
-
Homo sapiens
1.1.1.284
Hg2+
-
Homo sapiens
1.1.1.284
NAD+
competitive, nonlinear inhibitor, when the concentration of NADH varies at constant S-formylglutathione concentration, noncompetitive inhibitor when the concentration of S-formylglutathione is varied at constant NADH concentration
Homo sapiens
1.1.1.284
p-hydroxymercuribenzoate
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.0042
-
NADH
pH 5.7
Homo sapiens
1.1.1.284
0.008
-
glutathione
pH 8
Homo sapiens
1.1.1.284
0.009
-
formaldehyde
pH 8
Homo sapiens
1.1.1.284
0.045
-
NADPH
pH 5.7
Homo sapiens
1.1.1.284
0.3
-
S-formylglutathione
pH 5.7, with NADH as cofactor
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.284
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.284
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.284
3.17
-
-
Homo sapiens
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.284
0°C, half-life in absence of a stabilizer is a few h, in presence of 1 mM NAD+ or 40% v/v glycerol the half life is about 1 month, 0.5 mM NADH is even more effective
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
3-ethoxy-2-hydroxybutyraldehyde + glutathione + NAD+
weak activity
287870
Homo sapiens
S-(3-ethoxy-2-hydroxybutyryl)-glutathione + NADH + H+
-
-
-
?
1.1.1.284
formaldehyde + glutathione + NAD+
at pH 8 the rate of the reverse reaction with S-formylglutathione and NADH is about the same as that of the forward reaction with formaldehyde, glutathione and NAD+. At pH 5.7 the rate of the reverse reaction with S-formylglutathione and NADH is 3.9times and with S-formylglutathione and NADPH 2.0times, that of the forward reaction rate with NAD+ at pH 8.0
287870
Homo sapiens
S-formylglutathione + NADH + H+
-
287870
Homo sapiens
?
1.1.1.284
formaldehyde + glutathione + NAD+
at pH 8 the rate of the reverse reaction with S-formylglutathione and NADH is about the same as that of the forward reaction with formaldehyde, glutathione and NAD+. At pH 5.7 the rate of the reverse reaction with S-formylglutathione and NADH is 3.9times and with S-formylglutathione and NADPH 2.0times, that of the forward reaction rate with NAD+ at pH 8.0
287870
Homo sapiens
S-formylglutathione + NADH + H+
-
287870
Homo sapiens
r
1.1.1.284
formaldehyde + glutathione + NADP+
-
287870
Homo sapiens
S-formylglutathione + NADPH + H+
-
287870
Homo sapiens
r
1.1.1.284
glyoxal + glutathione + NAD+
weak activity
287870
Homo sapiens
S-oxoacetylglutathione + NADH
-
-
-
?
1.1.1.284
hydroxypyruvaldehyde + glutathione + NAD+
weak activity
287870
Homo sapiens
S-hydroxypyruvylglutathione + NADH
-
-
-
?
1.1.1.284
methylglyoxal + glutathione + NAD+
85% of the activity with formaldehyde
287870
Homo sapiens
S-pyruvylglutathione + NADH
-
-
-
?
1.1.1.284
S-formylglutathione + NADH
-
287870
Homo sapiens
formaldehyde + glutathione + NAD+
-
287870
Homo sapiens
r
1.1.1.284
S-formylglutathione + NADPH
-
287870
Homo sapiens
formaldehyde + glutathione + NADP+
-
287870
Homo sapiens
r
1.1.1.284
S-pyruvylglutathione + NADH
-
287870
Homo sapiens
methylglyoxal + glutathione + NAD+
-
287870
Homo sapiens
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.284
5.7
-
reaction with S-formylglutathione and NADH
Homo sapiens
1.1.1.284
8
-
oxidation of formaldehyde
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Homo sapiens
1.1.1.284
NADH
-
Homo sapiens
1.1.1.284
NADP+
at pH 8 the Km-value is about 1000times greater than that for NAD+. At pH 6 NADP+ is used better but this reaction appears to be without physiological significance
Homo sapiens
1.1.1.284
NADPH
-
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Homo sapiens
1.1.1.284
NADH
-
Homo sapiens
1.1.1.284
NADP+
at pH 8 the Km-value is about 1000times greater than that for NAD+. At pH 6 NADP+ is used better but this reaction appears to be without physiological significance
Homo sapiens
1.1.1.284
NADPH
-
Homo sapiens
General Stability (protein specific)
EC Number
General Stability
Organism
1.1.1.284
NAD+ and NADH protect from denaturation by high temperatures, acid pH and storage at 0°C
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.284
ADP-ribose
-
Homo sapiens
1.1.1.284
Alpha-NAD+
-
Homo sapiens
1.1.1.284
Hg2+
-
Homo sapiens
1.1.1.284
NAD+
competitive, nonlinear inhibitor, when the concentration of NADH varies at constant S-formylglutathione concentration, noncompetitive inhibitor when the concentration of S-formylglutathione is varied at constant NADH concentration
Homo sapiens
1.1.1.284
p-hydroxymercuribenzoate
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.0042
-
NADH
pH 5.7
Homo sapiens
1.1.1.284
0.008
-
glutathione
pH 8
Homo sapiens
1.1.1.284
0.009
-
formaldehyde
pH 8
Homo sapiens
1.1.1.284
0.045
-
NADPH
pH 5.7
Homo sapiens
1.1.1.284
0.3
-
S-formylglutathione
pH 5.7, with NADH as cofactor
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.284
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.284
3.17
-
-
Homo sapiens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.284
0°C, half-life in absence of a stabilizer is a few h, in presence of 1 mM NAD+ or 40% v/v glycerol the half life is about 1 month, 0.5 mM NADH is even more effective
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
3-ethoxy-2-hydroxybutyraldehyde + glutathione + NAD+
weak activity
287870
Homo sapiens
S-(3-ethoxy-2-hydroxybutyryl)-glutathione + NADH + H+
-
-
-
?
1.1.1.284
formaldehyde + glutathione + NAD+
at pH 8 the rate of the reverse reaction with S-formylglutathione and NADH is about the same as that of the forward reaction with formaldehyde, glutathione and NAD+. At pH 5.7 the rate of the reverse reaction with S-formylglutathione and NADH is 3.9times and with S-formylglutathione and NADPH 2.0times, that of the forward reaction rate with NAD+ at pH 8.0
287870
Homo sapiens
S-formylglutathione + NADH + H+
-
287870
Homo sapiens
?
1.1.1.284
formaldehyde + glutathione + NAD+
at pH 8 the rate of the reverse reaction with S-formylglutathione and NADH is about the same as that of the forward reaction with formaldehyde, glutathione and NAD+. At pH 5.7 the rate of the reverse reaction with S-formylglutathione and NADH is 3.9times and with S-formylglutathione and NADPH 2.0times, that of the forward reaction rate with NAD+ at pH 8.0
287870
Homo sapiens
S-formylglutathione + NADH + H+
-
287870
Homo sapiens
r
1.1.1.284
formaldehyde + glutathione + NADP+
-
287870
Homo sapiens
S-formylglutathione + NADPH + H+
-
287870
Homo sapiens
r
1.1.1.284
glyoxal + glutathione + NAD+
weak activity
287870
Homo sapiens
S-oxoacetylglutathione + NADH
-
-
-
?
1.1.1.284
hydroxypyruvaldehyde + glutathione + NAD+
weak activity
287870
Homo sapiens
S-hydroxypyruvylglutathione + NADH
-
-
-
?
1.1.1.284
methylglyoxal + glutathione + NAD+
85% of the activity with formaldehyde
287870
Homo sapiens
S-pyruvylglutathione + NADH
-
-
-
?
1.1.1.284
S-formylglutathione + NADH
-
287870
Homo sapiens
formaldehyde + glutathione + NAD+
-
287870
Homo sapiens
r
1.1.1.284
S-formylglutathione + NADPH
-
287870
Homo sapiens
formaldehyde + glutathione + NADP+
-
287870
Homo sapiens
r
1.1.1.284
S-pyruvylglutathione + NADH
-
287870
Homo sapiens
methylglyoxal + glutathione + NAD+
-
287870
Homo sapiens
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.284
5.7
-
reaction with S-formylglutathione and NADH
Homo sapiens
1.1.1.284
8
-
oxidation of formaldehyde
Homo sapiens