BRENDA - Enzyme Database

Microbial oxidation of methanol: purification and properties of formaldehyde dehydrogenase from a Pichia sp. NRRL-Y-11328

Patel, R.N.; Hou, C.T.; Derelanko, P.; Arch. Biochem. Biophys. 221, 135-142 (1983)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.284
1,10-phenanthroline
1 mM, 30% inhibition
Komagataella pastoris
1.1.1.284
2,2'-dipyridyl
1 mM, 20% inhibition
Komagataella pastoris
1.1.1.284
Cd2+
1 mM, complete inhibition
Komagataella pastoris
1.1.1.284
Cu2+
-
Komagataella pastoris
1.1.1.284
Hg2+
1 mM, complete inhibition
Komagataella pastoris
1.1.1.284
imidazole
1 mM, 32% inhibition
Komagataella pastoris
1.1.1.284
iodoacetamide
1 mM, 20% inhibition
Komagataella pastoris
1.1.1.284
NEM
1 mM, 35% inhibition
Komagataella pastoris
1.1.1.284
PCMB
1 mM, complete inhibition
Komagataella pastoris
1.1.1.284
Zn2+
1 mM, 40% inhibition
Komagataella pastoris
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.12
-
NAD+
-
Komagataella pastoris
1.1.1.284
0.16
-
reduced glutathione
-
Komagataella pastoris
1.1.1.284
0.25
-
NADH
-
Komagataella pastoris
1.1.1.284
0.31
-
formaldehyde
-
Komagataella pastoris
1.1.1.284
0.6
-
S-formylglutathione
-
Komagataella pastoris
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.284
41000
-
2 * 41000, SDS-PAGE
Komagataella pastoris
1.1.1.284
84000
-
gel filtration
Komagataella pastoris
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.284
Komagataella pastoris
-
NRRL-Y-11328
-
1.1.1.284
Komagataella pastoris NRRL-Y-11328
-
NRRL-Y-11328
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.284
-
Komagataella pastoris
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.284
2090
-
-
Komagataella pastoris
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
formaldehyde + glutathione + NAD+
-
287867
Komagataella pastoris
S-formylglutathione + NADH + H+
-
287867
Komagataella pastoris
r
1.1.1.284
formaldehyde + glutathione + NAD+
-
287867
Komagataella pastoris NRRL-Y-11328
S-formylglutathione + NADH + H+
-
287867
Komagataella pastoris NRRL-Y-11328
r
1.1.1.284
methylglyoxal + glutathione + NAD+
-
287867
Komagataella pastoris
S-pyruvylglutathione + NADH
-
-
-
?
1.1.1.284
methylglyoxal + glutathione + NAD+
-
287867
Komagataella pastoris NRRL-Y-11328
S-pyruvylglutathione + NADH
-
-
-
?
1.1.1.284
S-formylglutathione + NADH
-
287867
Komagataella pastoris
formaldehyde + glutathione + NAD+
-
287867
Komagataella pastoris
r
1.1.1.284
S-formylglutathione + NADH
-
287867
Komagataella pastoris NRRL-Y-11328
formaldehyde + glutathione + NAD+
-
287867
Komagataella pastoris NRRL-Y-11328
r
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.284
dimer
2 * 41000, SDS-PAGE
Komagataella pastoris
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.284
8
-
oxidation of formaldehyde
Komagataella pastoris
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.284
7
9
pH 7.0: about 55% of maximal activity, pH 9.0: about 90% of maximal activity
Komagataella pastoris
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Komagataella pastoris
1.1.1.284
NADH
-
Komagataella pastoris
1.1.1.284
NADP+
no activity with NADP+
Komagataella pastoris
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Komagataella pastoris
1.1.1.284
NADH
-
Komagataella pastoris
1.1.1.284
NADP+
no activity with NADP+
Komagataella pastoris
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.284
1,10-phenanthroline
1 mM, 30% inhibition
Komagataella pastoris
1.1.1.284
2,2'-dipyridyl
1 mM, 20% inhibition
Komagataella pastoris
1.1.1.284
Cd2+
1 mM, complete inhibition
Komagataella pastoris
1.1.1.284
Cu2+
-
Komagataella pastoris
1.1.1.284
Hg2+
1 mM, complete inhibition
Komagataella pastoris
1.1.1.284
imidazole
1 mM, 32% inhibition
Komagataella pastoris
1.1.1.284
iodoacetamide
1 mM, 20% inhibition
Komagataella pastoris
1.1.1.284
NEM
1 mM, 35% inhibition
Komagataella pastoris
1.1.1.284
PCMB
1 mM, complete inhibition
Komagataella pastoris
1.1.1.284
Zn2+
1 mM, 40% inhibition
Komagataella pastoris
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.12
-
NAD+
-
Komagataella pastoris
1.1.1.284
0.16
-
reduced glutathione
-
Komagataella pastoris
1.1.1.284
0.25
-
NADH
-
Komagataella pastoris
1.1.1.284
0.31
-
formaldehyde
-
Komagataella pastoris
1.1.1.284
0.6
-
S-formylglutathione
-
Komagataella pastoris
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.284
41000
-
2 * 41000, SDS-PAGE
Komagataella pastoris
1.1.1.284
84000
-
gel filtration
Komagataella pastoris
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.284
-
Komagataella pastoris
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.284
2090
-
-
Komagataella pastoris
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
formaldehyde + glutathione + NAD+
-
287867
Komagataella pastoris
S-formylglutathione + NADH + H+
-
287867
Komagataella pastoris
r
1.1.1.284
formaldehyde + glutathione + NAD+
-
287867
Komagataella pastoris NRRL-Y-11328
S-formylglutathione + NADH + H+
-
287867
Komagataella pastoris NRRL-Y-11328
r
1.1.1.284
methylglyoxal + glutathione + NAD+
-
287867
Komagataella pastoris
S-pyruvylglutathione + NADH
-
-
-
?
1.1.1.284
methylglyoxal + glutathione + NAD+
-
287867
Komagataella pastoris NRRL-Y-11328
S-pyruvylglutathione + NADH
-
-
-
?
1.1.1.284
S-formylglutathione + NADH
-
287867
Komagataella pastoris
formaldehyde + glutathione + NAD+
-
287867
Komagataella pastoris
r
1.1.1.284
S-formylglutathione + NADH
-
287867
Komagataella pastoris NRRL-Y-11328
formaldehyde + glutathione + NAD+
-
287867
Komagataella pastoris NRRL-Y-11328
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.284
dimer
2 * 41000, SDS-PAGE
Komagataella pastoris
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.284
8
-
oxidation of formaldehyde
Komagataella pastoris
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.284
7
9
pH 7.0: about 55% of maximal activity, pH 9.0: about 90% of maximal activity
Komagataella pastoris