EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.284 | 1,10-phenanthroline | 1 mM, 30% inhibition | Komagataella pastoris | |
1.1.1.284 | 2,2'-dipyridyl | 1 mM, 20% inhibition | Komagataella pastoris | |
1.1.1.284 | Cd2+ | 1 mM, complete inhibition | Komagataella pastoris | |
1.1.1.284 | Cu2+ | - |
Komagataella pastoris | |
1.1.1.284 | Hg2+ | 1 mM, complete inhibition | Komagataella pastoris | |
1.1.1.284 | imidazole | 1 mM, 32% inhibition | Komagataella pastoris | |
1.1.1.284 | iodoacetamide | 1 mM, 20% inhibition | Komagataella pastoris | |
1.1.1.284 | NEM | 1 mM, 35% inhibition | Komagataella pastoris | |
1.1.1.284 | PCMB | 1 mM, complete inhibition | Komagataella pastoris | |
1.1.1.284 | Zn2+ | 1 mM, 40% inhibition | Komagataella pastoris |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.284 | 0.12 | - |
NAD+ | - |
Komagataella pastoris | |
1.1.1.284 | 0.16 | - |
reduced glutathione | - |
Komagataella pastoris | |
1.1.1.284 | 0.25 | - |
NADH | - |
Komagataella pastoris | |
1.1.1.284 | 0.31 | - |
formaldehyde | - |
Komagataella pastoris | |
1.1.1.284 | 0.6 | - |
S-formylglutathione | - |
Komagataella pastoris |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.284 | 41000 | - |
2 * 41000, SDS-PAGE | Komagataella pastoris |
1.1.1.284 | 84000 | - |
gel filtration | Komagataella pastoris |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.284 | Komagataella pastoris | - |
NRRL-Y-11328 | - |
1.1.1.284 | Komagataella pastoris NRRL-Y-11328 | - |
NRRL-Y-11328 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.284 | - |
Komagataella pastoris |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.284 | 2090 | - |
- |
Komagataella pastoris |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.284 | formaldehyde + glutathione + NAD+ | - |
Komagataella pastoris | S-formylglutathione + NADH + H+ | - |
r | |
1.1.1.284 | formaldehyde + glutathione + NAD+ | - |
Komagataella pastoris NRRL-Y-11328 | S-formylglutathione + NADH + H+ | - |
r | |
1.1.1.284 | methylglyoxal + glutathione + NAD+ | - |
Komagataella pastoris | S-pyruvylglutathione + NADH | - |
? | |
1.1.1.284 | methylglyoxal + glutathione + NAD+ | - |
Komagataella pastoris NRRL-Y-11328 | S-pyruvylglutathione + NADH | - |
? | |
1.1.1.284 | S-formylglutathione + NADH | - |
Komagataella pastoris | formaldehyde + glutathione + NAD+ | - |
r | |
1.1.1.284 | S-formylglutathione + NADH | - |
Komagataella pastoris NRRL-Y-11328 | formaldehyde + glutathione + NAD+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.284 | dimer | 2 * 41000, SDS-PAGE | Komagataella pastoris |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.284 | 8 | - |
oxidation of formaldehyde | Komagataella pastoris |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.284 | 7 | 9 | pH 7.0: about 55% of maximal activity, pH 9.0: about 90% of maximal activity | Komagataella pastoris |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.284 | NAD+ | - |
Komagataella pastoris | |
1.1.1.284 | NADH | - |
Komagataella pastoris | |
1.1.1.284 | NADP+ | no activity with NADP+ | Komagataella pastoris |