BRENDA - Enzyme Database

Formaldehyde dehydrogenase

Rose, Z.B.; Racker, E.; Methods Enzymol. 9, 357-360 (1966)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.284
formaldehyde
-
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.068
-
NAD+
-
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.284
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.284
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.284
0.64
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
formaldehyde + glutathione + NAD+
-
287864
Saccharomyces cerevisiae
S-formylglutathione + NADH + H+
-
287864
Saccharomyces cerevisiae
r
1.1.1.284
glyoxal + glutathione + NAD+
-
287864
Saccharomyces cerevisiae
S-oxoacetylglutathione + NADH
-
-
-
?
1.1.1.284
methylglyoxal + glutathione + NAD+
-
287864
Saccharomyces cerevisiae
S-pyruvylglutathione + NADH
-
-
-
?
1.1.1.284
S-formylglutathione + NADH
-
287864
Saccharomyces cerevisiae
formaldehyde + glutathione + NAD+
-
287864
Saccharomyces cerevisiae
r
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.284
8
-
-
Saccharomyces cerevisiae
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.284
6
8.5
active from pH 6.0 to pH 8.5
Saccharomyces cerevisiae
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Saccharomyces cerevisiae
1.1.1.284
NADH
-
Saccharomyces cerevisiae
1.1.1.284
NADP+
no activity with NADP+
Saccharomyces cerevisiae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.284
NAD+
-
Saccharomyces cerevisiae
1.1.1.284
NADH
-
Saccharomyces cerevisiae
1.1.1.284
NADP+
no activity with NADP+
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.284
formaldehyde
-
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.284
0.068
-
NAD+
-
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.284
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.284
0.64
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.284
formaldehyde + glutathione + NAD+
-
287864
Saccharomyces cerevisiae
S-formylglutathione + NADH + H+
-
287864
Saccharomyces cerevisiae
r
1.1.1.284
glyoxal + glutathione + NAD+
-
287864
Saccharomyces cerevisiae
S-oxoacetylglutathione + NADH
-
-
-
?
1.1.1.284
methylglyoxal + glutathione + NAD+
-
287864
Saccharomyces cerevisiae
S-pyruvylglutathione + NADH
-
-
-
?
1.1.1.284
S-formylglutathione + NADH
-
287864
Saccharomyces cerevisiae
formaldehyde + glutathione + NAD+
-
287864
Saccharomyces cerevisiae
r
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.284
8
-
-
Saccharomyces cerevisiae
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.284
6
8.5
active from pH 6.0 to pH 8.5
Saccharomyces cerevisiae