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Literature summary extracted from
- Crystal structure of the 100 kDa arsenite oxidase from Alcaligenes faecalis in two crystal forms at 1.64 A and 2.03 A (2001), Structure, 9, 125-132.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.20.99.1 | additional information | induction by arsenite | Alcaligenes faecalis |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.20.9.1 | - |
Alcaligenes faecalis |
| 1.20.9.1 | crystal structure of arsenite oxidase to 2.03A in a P21 crystal form with two molecules in the asymmetric unit and to 1.64 A in a P1 crystal form with four molecules in the asymmetric unit. The large subunit contains a Mo site, consisting of a Mo atom bound to two pterin cofactors, and a [3Fe-4S] cluster. The small subunit contains a Rieske-type [2Fe-2S] site | Alcaligenes faecalis |
| 1.20.99.1 | X-ray diffraction structure determination and analysis of 2 different crystal forms at 1.64-2.03 A using multiple isomorphous replacement with anomalous scattering and multiple-wavelength anomalous dispersion methods | Alcaligenes faecalis |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.20.9.1 | Fe | contains a [3Fe-4S] cluster and a Rieske-type [2Fe-2S] site | Alcaligenes faecalis |  |
| 1.20.9.1 | Iron | the large subunit contains a [3Fe-4S] cluster. The small subunit contains a Rieske-type [2Fe-2S] site | Alcaligenes faecalis | |
| 1.20.9.1 | Molybdenum | the large subunit contains a Mo site, consisting of a Mo atom bound to two pterin cofactors | Alcaligenes faecalis | |
| 1.20.99.1 | Iron | enzyme is a MoFe protein, Mo atom bound to 2 pterin cofactors and a [3Fe4S] cluster, binding site on the large subunit, the small subunit contains a binding site for a Rieske-type [2Fe2S] cluster | Alcaligenes faecalis | |
| 1.20.99.1 | Molybdenum | enzyme is a MoFe protein, Mo atom bound to 2 pterin cofactors and a [3Fe4S] cluster, binding site on the large subunit, the Mo atom is not covalently bound to the polypeptide | Alcaligenes faecalis | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.20.99.1 | arsenite + acceptor | Alcaligenes faecalis | detoxification of arsenite to the less toxic arsenate prior to methylation | arsenate + reduced acceptor | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.20.9.1 | Alcaligenes faecalis | - |
- |
- |
| 1.20.9.1 | Alcaligenes faecalis | Q7SIF3 | small subunit aoxA | - |
| 1.20.9.1 | Alcaligenes faecalis | Q7SIF4 | large subunit aoxB | - |
| 1.20.9.1 | Alcaligenes faecalis NCIB 8687 | Q7SIF3 | small subunit aoxA | - |
| 1.20.9.1 | Alcaligenes faecalis NCIB 8687 | Q7SIF4 | large subunit aoxB | - |
| 1.20.99.1 | Alcaligenes faecalis | - |
NCIB 8687 | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.20.9.1 | arsenite + H2O + azurin(oxidized) | - |
Alcaligenes faecalis | arsenate + azurin(reduced) | - |
ir | |
| 1.20.99.1 | arsenite + acceptor | - |
Alcaligenes faecalis | arsenate + reduced acceptor | - |
? | |
| 1.20.99.1 | arsenite + acceptor | detoxification of arsenite to the less toxic arsenate prior to methylation | Alcaligenes faecalis | arsenate + reduced acceptor | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.20.9.1 | More | heterodimer | Alcaligenes faecalis |
| 1.20.99.1 | dimer | large subunit of 825 amino acid residues and a small subunit of 134 amino acid residues | Alcaligenes faecalis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.20.9.1 | aoxA | - |
Alcaligenes faecalis |
| 1.20.9.1 | aoxB | - |
Alcaligenes faecalis |
| 1.20.99.1 | arsenite oxidase | - |
Alcaligenes faecalis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.20.9.1 | iron-sulfur centre | the large subunit contains a [3Fe-4S] cluster. The small subunit contains a Rieske-type [2Fe-2S] site | Alcaligenes faecalis | |
| 1.20.9.1 | molybdopterin | the large subunit contains a Mo site, consisting of a Mo atom bound to two pterin cofactors | Alcaligenes faecalis | |
| 1.20.99.1 | Pterin molybdenum cofactor | enzyme is a MoFe protein, Mo atom bound to 2 pterin cofactors and a [3Fe4S] cluster, binding site on the large subunit | Alcaligenes faecalis | |
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