BRENDA - Enzyme Database

Chemical and functional properties of the native and reconstituted forms of the membrane-bound, aerobic glycerol-3-phosphate dehydrogenase of Escherichia coli

Schryvers, A.; Lohmeier, E.; Weiner, J.H.; J. Biol. Chem. 253, 783-788 (1978)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.5.3
detergent
detergent-depleted enzyme requires exogenous phospholipid or nondenaturing detergent for activity
Escherichia coli
1.1.5.3
Phospholipid
detergent-depleted enzyme requires exogenous phospholipid or nondenaturing detergent for activity
Escherichia coli
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.5.3
1,10-phenanthroline
-
Escherichia coli
1.1.5.3
D-2-Phosphoglyceric acid
-
Escherichia coli
1.1.5.3
D-3-phosphoglyceric acid
-
Escherichia coli
1.1.5.3
dihydroxyacetone phosphate
-
Escherichia coli
1.1.5.3
NEM
-
Escherichia coli
1.1.5.3
PCMB
-
Escherichia coli
1.1.5.3
phosphoenolpyruvate
-
Escherichia coli
1.1.5.3
Phosphoglycolic acid
-
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.5.3
membrane
-
Escherichia coli
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.5.3
Ca2+
-
Escherichia coli
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.5.3
58000
-
2 * 58000, SDS-PAGE
Escherichia coli
1.1.5.3
130000
-
gel filtration
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.5.3
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.5.3
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.5.3
sn-glycerol 3-phosphate + 2,6-dichlorophenolindophenol
-
287818
Escherichia coli
glycerone phosphate + reduced 2,6-dichlorophenylindophenol
-
-
-
?
1.1.5.3
sn-glycerol 3-phosphate + ferricyanide
-
287818
Escherichia coli
glycerone phosphate + ferrocyanide
-
-
-
?
1.1.5.3
sn-glycerol 3-phosphate + menadione
-
287818
Escherichia coli
glycerone phosphate + reduced menadione
-
-
-
?
1.1.5.3
sn-glycerol 3-phosphate + methylene blue
-
287818
Escherichia coli
glycerone phosphate + reduced methylene blue
-
-
-
?
1.1.5.3
sn-glycerol 3-phosphate + phenazine methosulfate
-
287818
Escherichia coli
glycerone phosphate + reduced phenazine methosulfate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.5.3
dimer
2 * 58000, SDS-PAGE
Escherichia coli
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.5.3
44
-
-
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.5.3
7
9.5
-
Escherichia coli
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.5.3
FAD
contains 0.4 mol of FAD per protein monomer of 58000 Da
Escherichia coli
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.5.3
detergent
detergent-depleted enzyme requires exogenous phospholipid or nondenaturing detergent for activity
Escherichia coli
1.1.5.3
Phospholipid
detergent-depleted enzyme requires exogenous phospholipid or nondenaturing detergent for activity
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.5.3
FAD
contains 0.4 mol of FAD per protein monomer of 58000 Da
Escherichia coli
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.5.3
1,10-phenanthroline
-
Escherichia coli
1.1.5.3
D-2-Phosphoglyceric acid
-
Escherichia coli
1.1.5.3
D-3-phosphoglyceric acid
-
Escherichia coli
1.1.5.3
dihydroxyacetone phosphate
-
Escherichia coli
1.1.5.3
NEM
-
Escherichia coli
1.1.5.3
PCMB
-
Escherichia coli
1.1.5.3
phosphoenolpyruvate
-
Escherichia coli
1.1.5.3
Phosphoglycolic acid
-
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.5.3
membrane
-
Escherichia coli
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.5.3
Ca2+
-
Escherichia coli
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.5.3
58000
-
2 * 58000, SDS-PAGE
Escherichia coli
1.1.5.3
130000
-
gel filtration
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.5.3
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.5.3
sn-glycerol 3-phosphate + 2,6-dichlorophenolindophenol
-
287818
Escherichia coli
glycerone phosphate + reduced 2,6-dichlorophenylindophenol
-
-
-
?
1.1.5.3
sn-glycerol 3-phosphate + ferricyanide
-
287818
Escherichia coli
glycerone phosphate + ferrocyanide
-
-
-
?
1.1.5.3
sn-glycerol 3-phosphate + menadione
-
287818
Escherichia coli
glycerone phosphate + reduced menadione
-
-
-
?
1.1.5.3
sn-glycerol 3-phosphate + methylene blue
-
287818
Escherichia coli
glycerone phosphate + reduced methylene blue
-
-
-
?
1.1.5.3
sn-glycerol 3-phosphate + phenazine methosulfate
-
287818
Escherichia coli
glycerone phosphate + reduced phenazine methosulfate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.5.3
dimer
2 * 58000, SDS-PAGE
Escherichia coli
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.5.3
44
-
-
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.5.3
7
9.5
-
Escherichia coli