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Literature summary extracted from

  • Schryvers, A.; Lohmeier, E.; Weiner, J.H.
    Chemical and functional properties of the native and reconstituted forms of the membrane-bound, aerobic glycerol-3-phosphate dehydrogenase of Escherichia coli (1978), J. Biol. Chem., 253, 783-788.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.5.3 detergent detergent-depleted enzyme requires exogenous phospholipid or nondenaturing detergent for activity Escherichia coli
1.1.5.3 Phospholipid detergent-depleted enzyme requires exogenous phospholipid or nondenaturing detergent for activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.5.3 1,10-phenanthroline
-
 Escherichia coli
1.1.5.3 D-2-Phosphoglyceric acid
-
 Escherichia coli
1.1.5.3 D-3-phosphoglyceric acid
-
 Escherichia coli
1.1.5.3 dihydroxyacetone phosphate
-
 Escherichia coli
1.1.5.3 NEM
-
 Escherichia coli
1.1.5.3 PCMB
-
 Escherichia coli
1.1.5.3 phosphoenolpyruvate
-
 Escherichia coli
1.1.5.3 Phosphoglycolic acid
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.3 membrane
-
 Escherichia coli 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.5.3 Ca2+
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.3 58000
-
 2 * 58000, SDS-PAGE Escherichia coli
1.1.5.3 130000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.3 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.3
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.3 sn-glycerol 3-phosphate + 2,6-dichlorophenolindophenol
-
 Escherichia coli glycerone phosphate + reduced 2,6-dichlorophenylindophenol
-
 ?
1.1.5.3 sn-glycerol 3-phosphate + ferricyanide
-
 Escherichia coli glycerone phosphate + ferrocyanide
-
 ?
1.1.5.3 sn-glycerol 3-phosphate + menadione
-
 Escherichia coli glycerone phosphate + reduced menadione
-
 ?
1.1.5.3 sn-glycerol 3-phosphate + methylene blue
-
 Escherichia coli glycerone phosphate + reduced methylene blue
-
 ?
1.1.5.3 sn-glycerol 3-phosphate + phenazine methosulfate
-
 Escherichia coli glycerone phosphate + reduced phenazine methosulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.3 dimer 2 * 58000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.3 44
-
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.3 7 9.5
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.3 FAD contains 0.4 mol of FAD per protein monomer of 58000 Da Escherichia coli