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Literature summary extracted from

  • Hildebrandt, E.F.; Preusch.P.C.; Patterson, J.L.; Suttie, J.W.
    Solubilization and characterization of vitamin K epoxide reductase from normal and warfarin-resistant rat liver microsomes (1984), Arch. Biochem. Biophys., 228, 480-492.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.4 dithiothreitol
-
 Rattus norvegicus

General Stability

EC Number General Stability Organism
1.17.4.4 Deriphat 160, detergent, solubilizes and inactivates Rattus norvegicus
1.17.4.4 high ionic strength inactivates during purification Rattus norvegicus
1.17.4.4 KCl stabilizes Rattus norvegicus
1.17.4.4 omission of salt improves storage stability Rattus norvegicus
1.17.4.4 physiological salt concentration stimulates Rattus norvegicus
1.17.4.4 Seriphat 160 stabilizes during purification Rattus norvegicus
1.17.4.4 sodium cholate, low-concentrated, stabilizes Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.4.4 cholate high concentration Rattus norvegicus
1.17.4.4 coumarin anticoagulants
-
 Rattus norvegicus
1.17.4.4 Deriphat 160 detergent, inactivation Rattus norvegicus
1.17.4.4 dithiothreitol high concentration Rattus norvegicus
1.17.4.4 warfarin
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.4.4 0.004
-
 vitamin K 2,3-epoxide
-
 Rattus norvegicus
1.17.4.4 0.16
-
 dithiothreitol
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.17.4.4 microsome
-
 Rattus norvegicus
-
-
1.17.4.4 microsome integral membrane protein Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.4.4 K2SO4 activation at high concentration Rattus norvegicus
1.17.4.4 KCl activation at high concentration Rattus norvegicus
1.17.4.4 LiCl activation at high concentration Rattus norvegicus
1.17.4.4 NaCl activation at high concentration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.4.4 2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone + 1,4-dithiothreitol Rattus norvegicus
-
 2-hydroxy-2-methyl-3-phytyl-2,3-dihydronaphthoquinone + oxidized dithiothreitol
-
 ?
1.17.4.4 2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone + 1,4-dithiothreitol Rattus norvegicus Holtzman
-
 2-hydroxy-2-methyl-3-phytyl-2,3-dihydronaphthoquinone + oxidized dithiothreitol
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.17.4.4 Ethanol up to 1% v/v, stable Rattus norvegicus
1.17.4.4 Ethanol 1% v/v, stable Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.4 Rattus norvegicus
-
-
-
1.17.4.4 Rattus norvegicus
-
 Holtzman strain
-
1.17.4.4 Rattus norvegicus Holtzman
-
 Holtzman strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.4.4
-
 Rattus norvegicus
1.17.4.4 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.4.4 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.4 2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone + 1,4-dithiothreitol
-
 Rattus norvegicus 2-hydroxy-2-methyl-3-phytyl-2,3-dihydronaphthoquinone + oxidized dithiothreitol
-
 ?
1.17.4.4 2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone + 1,4-dithiothreitol i.e. vitamin K 2,3-epoxide Rattus norvegicus 2-hydroxy-2-methyl-3-phytyl-2,3-dihydronaphthoquinone + oxidized dithiothreitol i.e. 3-hydroxy-2,3-dihydro-vitamin K ?
1.17.4.4 2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone + 1,4-dithiothreitol
-
 Rattus norvegicus Holtzman 2-hydroxy-2-methyl-3-phytyl-2,3-dihydronaphthoquinone + oxidized dithiothreitol
-
 ?
1.17.4.4 2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone + 1,4-dithiothreitol i.e. vitamin K 2,3-epoxide Rattus norvegicus Holtzman 2-hydroxy-2-methyl-3-phytyl-2,3-dihydronaphthoquinone + oxidized dithiothreitol i.e. 3-hydroxy-2,3-dihydro-vitamin K ?
1.17.4.4 vitamin K 2,3-epoxide + dithiothreitol
-
 Rattus norvegicus vitamin K + oxidized dithiothreitol
-
 ?
1.17.4.4 vitamin K 2,3-epoxide + dithiothreitol
-
 Rattus norvegicus Holtzman vitamin K + oxidized dithiothreitol
-
 ?
1.17.4.4 vitamin K 2,3-epoxide + reduced dithiothreitol
-
 Rattus norvegicus vitamin K quinone + oxidized dithiothreitol + H2O
-
 r
1.17.4.4 vitamin K 2,3-epoxide + reduced dithiothreitol
-
 Rattus norvegicus Holtzman vitamin K quinone + oxidized dithiothreitol + H2O
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.4.4 25
-
-
 Rattus norvegicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.17.4.4 7 35 about half-maximal activity at 7°C and 35°C Rattus norvegicus
1.17.4.4 18 35 about 55% of maximal activity at 18°C and 35°C Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.17.4.4
-
-
 considerably more stable than at 22°C Rattus norvegicus
1.17.4.4
-
-
 considerably stable Rattus norvegicus
1.17.4.4 15
-
 rapid inactivation Rattus norvegicus
1.17.4.4 22
-
 rapid inactivation Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.4.4 9
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.17.4.4 8.5 9.8 about half-maximal activity at pH 8.5 and 9.8 Rattus norvegicus