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Literature summary extracted from

  • Lee, S.; Rhee, H.; Tae, W.; Shin, J.; Park, B.
    Purification and characterization of cholesterol oxidase from Pseudomonas sp. and taxonomic study of the strain (1989), Appl. Microbiol. Biotechnol., 31, 542-546.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.6 Fe2+
-
 Pseudomonas sp.
1.1.3.6 Hg2+
-
 Pseudomonas sp.
1.1.3.6 Zn2+
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.6 0.2
-
 cholesterol
-
 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.3.6 Mn2+
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.6 56000
-
 SDS-PAGE, gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.6 cholesterol + O2 Pseudomonas sp.
-
 cholest-5-en-3-one + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.6 Pseudomonas sp.
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.3.6 cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Pseudomonas sp.
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.6 27.9
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.6 5alpha-cholestan-3beta-ol + O2
-
 Pseudomonas sp. 5alpha-cholestan-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2 enzyme specifically oxidizes 3beta-hydroxy groups in steroids Pseudomonas sp. cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Pseudomonas sp. cholest-5-en-3-one + H2O2
-
 ?
1.1.3.6 dihydrocholesterol + O2
-
 Pseudomonas sp. 5alpha-cholestan-3-one + H2O2
-
 ?
1.1.3.6 ergosterol + O2
-
 Pseudomonas sp. dehydroergosterol + H2O2
-
 ?
1.1.3.6 stigmastanol + O2
-
 Pseudomonas sp. stigmastanone + H2O2
-
 ?
1.1.3.6 stigmasterol + O2
-
 Pseudomonas sp. stigmasta-5,22-dien-3-one + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.6 monomer 1 * 56000, SDS-PAGE Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.6 70
-
 5 min, no loss of activity Pseudomonas sp.
1.1.3.6 70
-
 30 min, 15% loss of activity Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.6 7
-
-
 Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.6 5 9 33% of maximal activity at pH 5.0 and pH 9.0 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.6 5 8 60°C, 1 min, stable Pseudomonas sp.
1.1.3.6 10
-
 60°C, 1 min, 25% loss of activity Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.6 FAD exogenous addition increases activity Pseudomonas sp.