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Literature summary extracted from

  • Claiborne, A.
    Studies on the structure and mechanism of Streptococcus faecium L-alpha-glycerophosphate oxidase (1986), J. Biol. Chem., 261, 14398-14407.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.21 65000
-
 2 * 65000, SDS-PAGE Enterococcus faecium
1.1.3.21 123000
-
 strain ATCC 12755, sedimentation equilibrium Enterococcus faecium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.21 sn-glycerol-3-phosphate + O2 Enterococcus faecium
-
 glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.21 Enterococcus faecium
-
 ATCC 12755
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.21
-
 Enterococcus faecium

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.3.21 sn-glycerol 3-phosphate + O2 = glycerone phosphate + H2O2 mechanism Enterococcus faecium
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.21 62
-
 strain ATCC 12755 Enterococcus faecium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.21 sn-glycerol 3-phosphate + O2
-
 Enterococcus faecium glycerone phosphate + H2O2
-
 r
1.1.3.21 sn-glycerol-3-phosphate + O2
-
 Enterococcus faecium glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?
1.1.3.21 sn-glycerol-3-phosphate + O2 other electron acceptors: ferricyanide Enterococcus faecium glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?
1.1.3.21 sn-glycerol-3-phosphate + O2 ferricyanide, in absence of O2 Enterococcus faecium glycerone phosphate + H2O2 i.e. dihydroxyacetone phosphate ?

Subunits

EC Number Subunits Comment Organism
1.1.3.21 dimer 2 * 65000, SDS-PAGE Enterococcus faecium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.21 23.8
-
 sn-glycerol 3-phosphate strain ATCC 12755 Enterococcus faecium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.21 FAD prosthetic group Enterococcus faecium
1.1.3.21 FAD 2 mol bound per mol of enzyme Enterococcus faecium