EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.95 | - |
Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.95 | H344A | 50% inhibition at 0.8 mM L-serine | Escherichia coli |
1.1.1.95 | H344A/N364A | no inhibition by L-serine | Escherichia coli |
1.1.1.95 | N346A | 50% inhibition at 6 mM L-serine | Escherichia coli |
1.1.1.95 | N346A/H344A | no inhibition by L-serine | Escherichia coli |
1.1.1.95 | N346A/N364A | no inhibition by L-serine | Escherichia coli |
1.1.1.95 | N364A | 50% inhibition at 48 mM L-serine | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.95 | (R)-2-amino-1-propanol | slightly | Escherichia coli | |
1.1.1.95 | amino acids | - |
Escherichia coli | |
1.1.1.95 | beta-Alanine | slightly | Escherichia coli | |
1.1.1.95 | glycine | mutants show less to no inhibition | Escherichia coli | |
1.1.1.95 | L-alanine | native enzyme and mutant H344A | Escherichia coli | |
1.1.1.95 | L-serine | 50% inhibition at 0.008 mM L-serine; allosteric inhibition, regulates the pathway of serine biosynthesis by end product inhibition interacting with His344, Asn346 and Asn364 | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.95 | 0.038 | - |
alpha-ketoglutarate | apparent, double mutant H344A/N364A | Escherichia coli | |
1.1.1.95 | 0.042 | - |
alpha-ketoglutarate | apparent | Escherichia coli | |
1.1.1.95 | 0.044 | - |
alpha-ketoglutarate | apparent, mutant N346A | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.95 | 3-phosphoglycerate + NAD+ | Escherichia coli | allosteric inhibition by L-serine, L-serine regulates the pathway of serine biosynthesis by end product inhibition interacting with His344, Asn346 and Asn364, 1 serine binds per subunit | 3-phosphohydroxypyruvate + NADH | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.95 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.95 | - |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.95 | 3-phosphoglycerate + NAD+ | - |
Escherichia coli | 3-phosphohydroxypyruvate + NADH | - |
r | |
1.1.1.95 | 3-phosphoglycerate + NAD+ | allosteric inhibition by L-serine, L-serine regulates the pathway of serine biosynthesis by end product inhibition interacting with His344, Asn346 and Asn364, 1 serine binds per subunit | Escherichia coli | 3-phosphohydroxypyruvate + NADH | - |
r |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.95 | 0.128 | - |
alpha-ketoglutarate | - |
Escherichia coli | |
1.1.1.95 | 0.137 | - |
alpha-ketoglutarate | double mutant H344A/N364A | Escherichia coli | |
1.1.1.95 | 0.32 | - |
alpha-ketoglutarate | mutant N346A | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.95 | NAD+ | - |
Escherichia coli | |
1.1.1.95 | NADH | - |
Escherichia coli |