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Literature summary extracted from

  • Fickenscher, K.; Scheibe, R.
    Purification and properties of NADP-dependent malate dehydrogenase from pea leaves (1983), Biochim. Biophys. Acta, 749, 249-254.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.82 0.0145
-
 (S)-malate
-
 Pisum sativum
1.1.1.82 0.039
-
 NADPH
-
 Pisum sativum
1.1.1.82 0.048
-
 oxaloacetate
-
 Pisum sativum
1.1.1.82 0.063
-
 NADP+
-
 Pisum sativum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.82 Mg2+ not essential, stimulates up to 20%, optimal concentration is 10 mM MgCl2 Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.82 38500
-
 1 * 38500, enzyme exists as monomer, dimer and tetramer, SDS-PAGE Pisum sativum
1.1.1.82 38500
-
 2 * 38500, enzyme exists as monomer, dimer and tetramer, SDS-PAGE Pisum sativum
1.1.1.82 38500
-
 4 * 38500, enzyme exists as monomer, dimer and tetramer, SDS-PAGE Pisum sativum
1.1.1.82 40000
-
 monomer, gel filtration Pisum sativum
1.1.1.82 87000
-
 dimer, gel filtration Pisum sativum
1.1.1.82 163000
-
 tetramer, gel filtration Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.82 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.82
-
 Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.82 leaf
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.82 184
-
-
 Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.82 (S)-malate + NADP+
-
 Pisum sativum oxaloacetate + NADPH + H+
-
 r
1.1.1.82 oxaloacetate + NADPH
-
 Pisum sativum (S)-malate + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.82 dimer 2 * 38500, enzyme exists as monomer, dimer and tetramer, SDS-PAGE Pisum sativum
1.1.1.82 monomer 1 * 38500, enzyme exists as monomer, dimer and tetramer, SDS-PAGE Pisum sativum
1.1.1.82 More enzyme exists as monomer dimer and tetramer. At neutral pH, pH 7.0-8.6, the enzyme is tetrameric Pisum sativum
1.1.1.82 tetramer 4 * 38500, enzyme exists as monomer, dimer and tetramer, SDS-PAGE Pisum sativum