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Literature summary extracted from

  • Fernandez-Sousa, J.M.; Gavilanes, J.G.; Municio, A.M.; Perez-Aranda, A.
    L-glycerol-3-phosphate dehydrogenase from the insect Ceratitis capitata. Purfication, physicochemical and enzymic properties (1977), Biochim. Biophys. Acta, 481, 6-24.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.8 2-mercaptoethanol stabilizes during purification Ceratitis capitata

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.8 additional information high ionic strength above 0.03 M Ceratitis capitata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.8 0.018
-
 NADH
-
 Ceratitis capitata
1.1.1.8 0.26
-
 NAD+
-
 Ceratitis capitata
1.1.1.8 0.33
-
 dihydroxyacetone phosphate
-
 Ceratitis capitata
1.1.1.8 0.74
-
 sn-glycerol 3-phosphate
-
 Ceratitis capitata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.8 33500
-
 2 * 33500, SDS-PAGE Ceratitis capitata
1.1.1.8 72500
-
 69000-76000, gel filtration Ceratitis capitata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.8 sn-glycerol 3-phosphate + NAD+ Ceratitis capitata
-
 dihydroxyacetone phosphate + NADH + H+
-
 r
1.1.1.8 sn-glycerol 3-phosphate + NAD+ Ceratitis capitata Wiedemann
-
 dihydroxyacetone phosphate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.8 Ceratitis capitata
-
 mediterranean fruit fly
-
1.1.1.8 Ceratitis capitata Wiedemann
-
 mediterranean fruit fly
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.8
-
 Ceratitis capitata

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.8 198.9
-
-
 Ceratitis capitata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.8 sn-glycerol 3-phosphate + NAD+
-
 Ceratitis capitata dihydroxyacetone phosphate + NADH + H+
-
 r
1.1.1.8 sn-glycerol 3-phosphate + NAD+
-
 Ceratitis capitata Wiedemann dihydroxyacetone phosphate + NADH + H+
-
 r
1.1.1.8 sn-glycerol 3-phosphate + NAD+
-
 Ceratitis capitata glycerone phosphate + NADH + H+
-
 r
1.1.1.8 sn-glycerol 3-phosphate + NAD+
-
 Ceratitis capitata Wiedemann glycerone phosphate + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.8 dimer 2 * 33500, SDS-PAGE Ceratitis capitata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.8 31
-
-
 Ceratitis capitata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.8 20
-
 between pH 6.0 and 9.0, stable for 1 h Ceratitis capitata
1.1.1.8 30
-
 30 min stable, pH 6.6 Ceratitis capitata
1.1.1.8 45
-
 pH 6.6, 5 min stable Ceratitis capitata
1.1.1.8 55
-
 inactivation after 30 min Ceratitis capitata
1.1.1.8 60
-
 inactivation after 5 min Ceratitis capitata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.8 32
-
 sn-glycerol 3-phosphate per subunit of 33500 Da Ceratitis capitata
1.1.1.8 188
-
 dihydroxyacetone phosphate per subunit of 33500 Da Ceratitis capitata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.8 6.6
-
 optimum pH for NADH oxidation around pH 6.6 Ceratitis capitata
1.1.1.8 10
-
 optimum pH for NAD+ reduction about pH 10.0 Ceratitis capitata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.8 5.2 7.8 about half-maximal activity between pH 5.2 and 7.8, reduction of dihydroxyacetone phosphate Ceratitis capitata
1.1.1.8 8 11 about half-maximal activity at pH 8.0 and 11.0, oxidation of L-glycerol-3-phosphate Ceratitis capitata

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.8 5
-
 inactivation after 1 h at 20°C Ceratitis capitata
1.1.1.8 6 9 stable for 16 h at 20°C Ceratitis capitata
1.1.1.8 10
-
 inactivation after 1 h at 20°C Ceratitis capitata

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.8 NAD+
-
 Ceratitis capitata
1.1.1.8 NADH
-
 Ceratitis capitata