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Literature summary extracted from

  • Ostro, M.J.; Fondy, T.P.
    Isolation and characterization of multiple molecular forms of cytosolic NAD-linked glycerol-3-phosphate dehydrogenase from normal and neoplastic rabbit tissues (1977), J. Biol. Chem., 252, 5575-5583.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.8 p-chloromercuribenzoate 10 nM, strong inhibition Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.8 additional information
-
 additional information various Km-values of 4 isozymes of heart, muscle, liver and mammary gland Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.8 cytoplasm
-
 Oryctolagus cuniculus 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.8 37500
-
 x * 37500, isozyme from heart (II6.1), calculated by amino acid composition Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.8 sn-glycerol 3-phosphate + NAD+ Oryctolagus cuniculus favoured reaction of heart isozyme II6.1 dihydroxyacetone phosphate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.8 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.8 several isozymes, distinct in isoelectric points Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.8 Brown Pearce carcinoma cell
-
 Oryctolagus cuniculus
-
1.1.1.8 liver
-
 Oryctolagus cuniculus
-
1.1.1.8 mammary gland
-
 Oryctolagus cuniculus
-
1.1.1.8 muscle heart muscle Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.8 115
-
 heart Oryctolagus cuniculus
1.1.1.8 180
-
 mammary gland Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
1.1.1.8 4°C, 20% v/v, glycerol, purified stable Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.8 sn-glycerol 3-phosphate + NAD+
-
 Oryctolagus cuniculus dihydroxyacetone phosphate + NADH + H+
-
 r
1.1.1.8 sn-glycerol 3-phosphate + NAD+ favoured reaction of heart isozyme II6.1 Oryctolagus cuniculus dihydroxyacetone phosphate + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.8 ? x * 37500, isozyme from heart (II6.1), calculated by amino acid composition Oryctolagus cuniculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.8 48
-
 t1/2: 2 min, heart isozyme II6.1 Oryctolagus cuniculus
1.1.1.8 48
-
 t1/2: 2 min, brain isozyme I5.9 Oryctolagus cuniculus
1.1.1.8 48
-
 t1/2: 15 min, muscle isozyme I6.5 Oryctolagus cuniculus
1.1.1.8 50
-
 t1/2: 2 min in heart and 10 min in muscle, mammary gland and liver Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.8 additional information no activity with NADPH Oryctolagus cuniculus
1.1.1.8 additional information not NADP+ Oryctolagus cuniculus
1.1.1.8 NAD+
-
 Oryctolagus cuniculus
1.1.1.8 NADH
-
 Oryctolagus cuniculus