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Literature summary extracted from

  • Boronat, A.; Aguilar,J.
    Rhamnose-induced propanediol oxidoreductase in Escherichia coli: purification, properties and comparison with the fucose-induced enzyme (1979), J. Bacteriol., 140, 320-326.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.77 L-1,2-propanediol above 25 mM: substrate inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.77 0.035
-
 L-lactaldehyde pH 7.0 Escherichia coli
1.1.1.77 1.25
-
 L-1,2-propanediol pH 9.5 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.77 39000
-
 2 * 39000, SDS-PAGE Escherichia coli
1.1.1.77 76000
-
 gel permeation chromatography on a calibrated column of Ultrogel AcA 34 under denaturating conditions Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.77 (S)-propane-1,2-diol + NAD+ Escherichia coli
-
 (S)-lactaldehyde + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.77 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.77 crude extract, ammonium sulfate precipitation, heat treatment, ultrogel AcA 34 fractionation, DEAE-Sephadex column chromatography, second ultrogel AcA 34 fractionation Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.77 (R)-propane-1,2-diol + NAD+ acts only on L-isomer Escherichia coli (R)-lactaldehyde + NADH
-
 ?
1.1.1.77 (S)-propane-1,2-diol + NAD+
-
 Escherichia coli (S)-lactaldehyde + NADH
-
 r
1.1.1.77 (S)-propane-1,2-diol + NAD+ only L-propane-1,2-diol is oxidized Escherichia coli (S)-lactaldehyde + NADH
-
 ?
1.1.1.77 ethanol + NAD+
-
 Escherichia coli acetaldehyde + NADH
-
 r
1.1.1.77 ethylene glycol + NAD+
-
 Escherichia coli glycolaldehyde + NADH + H+
-
 r
1.1.1.77 glycerol + NAD+
-
 Escherichia coli DL-glyceraldehyde + NADH
-
 r
1.1.1.77 propanol + NAD+
-
 Escherichia coli propionaldehyde + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.77 dimer 2 * 39000, SDS-PAGE Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.77 6.5
-
 reduction of L-lactaldehyde Escherichia coli
1.1.1.77 9.5
-
 dehydrogenation of L-1,2-propanediol Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.77 4.5 10 reduction of lactaldehyde, pH 4.5: 50% of reductase activity maximum, pH 10: 10% of reductase activity maximum Escherichia coli
1.1.1.77 8 11 dehydrogenation of 1,2-propanediol, pH 8: 10% of dehydrogenase activity maximum, pH 11: 65% of dehydrogenase activity maximum Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.77 NAD+
-
 Escherichia coli
1.1.1.77 NADH
-
 Escherichia coli