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Literature summary extracted from
- A specific mannitol dehydrogenase from Lactobacillus brevis (1963), J. Biol. Chem., 238, 1598-1603.
No PubMed abstract available
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.1.1.67 | - |
Levilactobacillus brevis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.67 | 0.13 | - |
NADH | - |
Levilactobacillus brevis |  |
| 1.1.1.67 | 0.18 | 0.27 | NAD+ | - |
Levilactobacillus brevis | |
| 1.1.1.67 | 16.3 | 79.2 | D-fructose | - |
Levilactobacillus brevis | |
| 1.1.1.67 | 60 | - |
D-mannitol | - |
Levilactobacillus brevis | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.67 | D-mannitol + NAD+ | Levilactobacillus brevis | - |
D-fructose + NADH + H+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.67 | Levilactobacillus brevis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.67 | - |
Levilactobacillus brevis |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.67 | -16°C, 0.02 M sodium acetate buffer pH 6.0, 1 mM 2-mercaptoethanol, several weeks | Levilactobacillus brevis |
| 1.1.1.67 | 3°C, crystalline enzyme in ammonium sulfate, 2 months | Levilactobacillus brevis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.67 | D-mannitol + NAD+ | - |
Levilactobacillus brevis | D-fructose + NADH + H+ | - |
? | |
| 1.1.1.67 | D-mannitol + NAD+ | enzyme highly specific for D-mannitol and D-fructose | Levilactobacillus brevis | D-fructose + NADH + H+ | - |
r | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.67 | 5.3 | - |
D-fructose reduction | Levilactobacillus brevis |
| 1.1.1.67 | 8.6 | - |
D-mannitol oxidation | Levilactobacillus brevis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.67 | 5 | - |
not active below, D-mannitol oxidation | Levilactobacillus brevis |
| 1.1.1.67 | 8 | - |
not active above, D-fructose reduction | Levilactobacillus brevis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.67 | 6 | 6.5 | - |
Levilactobacillus brevis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.67 | NAD+ | - |
Levilactobacillus brevis | |
| 1.1.1.67 | NADH | - |
Levilactobacillus brevis | |
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Release 2023.1 (January 2023)
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