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Literature summary extracted from

  • Yamanaka, K.
    D-Mannitol dehydrogenase from Leuconostoc mesenteroides (1975), Methods Enzymol., 41B, 138-142.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.67
-
 Leuconostoc mesenteroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.67 0.01 0.079 NADH
-
 Leuconostoc mesenteroides
1.1.1.67 0.01 0.079 NADH
-
 Levilactobacillus brevis
1.1.1.67 0.18 0.27 NAD+
-
 Leuconostoc mesenteroides
1.1.1.67 0.18 0.27 NAD+
-
 Levilactobacillus brevis
1.1.1.67 0.29 21.8 D-mannitol
-
 Leuconostoc mesenteroides
1.1.1.67 0.29 21.8 D-mannitol
-
 Levilactobacillus brevis
1.1.1.67 16.3 79.2 D-fructose
-
 Leuconostoc mesenteroides
1.1.1.67 16.3 79.2 D-fructose
-
 Levilactobacillus brevis
1.1.1.67 60
-
 D-mannitol
-
 Levilactobacillus brevis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.67 132000 138000 gel filtration, sucrose density gradient centrifugation Leuconostoc mesenteroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.67 D-mannitol + NAD+ Leuconostoc mesenteroides
-
 D-fructose + NADH + H+
-
 ?
1.1.1.67 D-mannitol + NAD+ Levilactobacillus brevis
-
 D-fructose + NADH + H+
-
 ?
1.1.1.67 D-mannitol + NAD+ Lactobacillus gayonii
-
 D-fructose + NADH + H+
-
 ?
1.1.1.67 D-mannitol + NAD+ Lactobacillus pentoaceticus
-
 D-fructose + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.67 Lactobacillus gayonii
-
-
-
1.1.1.67 Lactobacillus pentoaceticus
-
-
-
1.1.1.67 Leuconostoc mesenteroides
-
-
-
1.1.1.67 Levilactobacillus brevis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.67
-
 Leuconostoc mesenteroides

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.67 cell culture glucose induces enzyme production Leuconostoc mesenteroides
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.67 393
-
-
 Leuconostoc mesenteroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.67 D-mannitol + NAD+
-
 Leuconostoc mesenteroides D-fructose + NADH + H+
-
 ?
1.1.1.67 D-mannitol + NAD+
-
 Leuconostoc mesenteroides D-fructose + NADH + H+
-
 r
1.1.1.67 D-mannitol + NAD+
-
 Levilactobacillus brevis D-fructose + NADH + H+
-
 ?
1.1.1.67 D-mannitol + NAD+
-
 Levilactobacillus brevis D-fructose + NADH + H+
-
 r
1.1.1.67 D-mannitol + NAD+
-
 Lactobacillus gayonii D-fructose + NADH + H+
-
 ?
1.1.1.67 D-mannitol + NAD+
-
 Lactobacillus pentoaceticus D-fructose + NADH + H+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.67 5.3
-
 D-fructose reduction Leuconostoc mesenteroides
1.1.1.67 5.3
-
 D-fructose reduction Levilactobacillus brevis
1.1.1.67 8.6
-
 D-mannitol oxidation Leuconostoc mesenteroides
1.1.1.67 8.6
-
 D-mannitol oxidation Levilactobacillus brevis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.67 5 9 at 35°C for 1 h Leuconostoc mesenteroides
1.1.1.67 6 8 at 35°C for 5 h Leuconostoc mesenteroides

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.67 NAD+
-
 Leuconostoc mesenteroides
1.1.1.67 NAD+
-
 Levilactobacillus brevis
1.1.1.67 NAD+
-
 Lactobacillus gayonii
1.1.1.67 NAD+
-
 Lactobacillus pentoaceticus
1.1.1.67 NADH
-
 Leuconostoc mesenteroides
1.1.1.67 NADH
-
 Levilactobacillus brevis
1.1.1.67 NADH
-
 Lactobacillus gayonii
1.1.1.67 NADH
-
 Lactobacillus pentoaceticus